Recovery of virus from cell culture using a hypertonic salt solution
AssigneeXenova Research Limited
InventorsMichael Johnston, Roderic O'Keeffe
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Abstract
A process of harvesting a herpesvirus from a cell culture infected therewith comprises treating said culture with a hypertonic aqueous salt solution to yield a virus suspension, e.g. to give improved yield of virus for live virus vaccine where otherwise cell-disruption might be used to harvest the virus by disrupting virus-infected cells. The harvesting step can be followed e.g. by nuclease treatment, diafiltration and lyophilisation.
Claims9
1:
- A process of harvesting a herpesvirus from a cell culture infected therewith, which comprises treating said culture with a hypertonic aqueous salt solution to yield a virus suspension.
2:
- A process according to claim 1, wherein the virus suspension is further treated to formulate it as a pharmaceutical preparation suitable for use as a virus vaccine.
3:
- A process according to claim 1, wherein the salt comprises sodium chloride at about 0.8 M concentration or above.
A process according to claim 1, wherein the harvesting salt solution is buffered at a pH about 7 and temperature about 34 deg.C. for the harvesting of herpes simplex virus.
A process according to claim 1, wherein the harvested preparation is then diluted or dia-filtered to approximately isotonic concentration.
A process according to claim 1 wherein the harvested virus preparation is treated with nuclease enzyme.
A process according to claim 1, wherein the harvested virus, after transfer to a desired carrier liquid, is frozen, lyophilized or otherwise stabilized.
A process according to claim 1, wherein the virus comprises herpes simplex virus type 2 (HSV-2), pseudorabies virus (PRV), turkey herpesvirus, or varicella zoster virus (VZV).
Dependencies are inferred from the claim text.