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US 2006/0141508 A1Published application
Cellular signaling pathway based assays, reagents and kits
OwnerMichelle Palmerinventor
Life of this patent
Estimating the term…
Abstract
The present invention is directed to an assay system, PAM (Pathway Assay Matrix) for screening drug to determine whether they are effective for treating or preventing a disease or disorder. This assay system takes into account that many diseases involve complex pathways, and measures the effect of a particular drug on the multiple nodes within the disease pathway of a particular disease or disorder.
Claims46
An assay system for screening drugs to determine whether they are effective for treating or preventing a disease comprising:
- a microarray plate;
- at least two primary cells, cell lines or cell lysates in six or more wells of said microarray plate wherein the primary cells, cell lines or cell lysates are chosen to represent a particular disease pathway for the disease for which drugs are being screened, with the caveat that only one primary cell, cell line or cell lysate is present in any single well, wherein said primary cells or cell lines are treated in a tissue culture plate well or vessel with at least one drug of interest prior to adding them, or a cell lysate obtained from the primary cell or cell line culture, to the wells of the microarray plate;
- and
- six or more reagents, at least one in each of the wells of said microarray plate occupied by a primary cell, cell line or cell lysate.
1 more element
A method of screening drugs for their effectiveness for treating or preventing a disease comprising:
- treating primary cells or cell lines in tissue culture plate wells or vessels with a drug of interest, wherein multiple primary cells or cell lines are treated, each in their own tissue culture plate well or vessel;
- optionally lysing the primary cells or cell lines;
- adding the treated primary cells, cell lines or cell lysates to six or more wells of a microarray plate, with the caveat that only one primary cell, cell line or cell lysate is present in any single well;
- adding six or more reagents to the wells of said microarray plate occupied by a primary cell, cell line or cell lysate, wherein at least one reagent is added to each of the wells of said microarray plate occupied by a primary cell, cell line or cell lysate;
- conducting an assay reaction for each well of said microarray plate, wherein each reagent and or assay is chosen to measure a particular node in the disease pathway for the disease for which drugs are being screened;
- and
- analyzing the effect the drug of interest has on each of the nodes of the disease pathway, thereby determining whether the drug of interest would be effective for treating or preventing the disease.
4 more elements
A method of screening drugs for their effectiveness for treating or preventing a disease comprising:
- treating primary cells or cell lines in tissue culture plate wells or vessels with a drug of interest, wherein multiple primary cells or cell lines are treated, each in their own tissue culture plate well or vessel;
- optionally lysing the primary cells or cell lines;
- adding six or more reagents to six or more wells of a microarray plate, wherein at least one reagent is added to each of the wells of said microarray plate occupied by a primary cell, cell line or cell lysate;
- adding the treated primary cells, cell lines or cell lysates to the six or more wells of a microarray plate containing a reagent, with the caveat that only one primary cell, cell line or cell lysate is present in any single well;
- conducting an assay reaction for each well of said microarray plate, wherein each reagent and or assay is chosen to measure a particular node in the disease pathway for the disease for which drugs are being screened;
- and
- analyzing the effect the drug of interest has on each of the nodes of the disease pathway, thereby determining whether the drug of interest would be effective for treating or preventing the disease.
4 more elements
A method of screening drugs for their effectiveness for treating or preventing a disease comprising:
- treating primary cells or cell lines in six or more tissue culture plate wells or vessels with a drug of interest, wherein multiple primary cells or cell lines are treated, each in their own tissue culture plate well or vessel;
- optionally lysing the primary cells or cell lines;
- adding six or more reagents to the six or more tissue culture plate wells or vessels, wherein at least one reagent is added to each of the tissue culture plate wells or vessels;
- adding the reagents and the treated primary cells, cell lines or cell lysates to six or more wells of a microarray plate, with the caveat that only one primary cell, cell line or cell lysate is present in any single well;
- conducting an assay reaction for each well of said microarray plate, wherein each reagent and or assay is chosen to measure a particular node in the disease pathway for the disease for which drugs are being screened;
- and
- analyzing the effect the drug of interest has on each of the nodes of the disease pathway, thereby determining whether the drug of interest would be effective for treating or preventing the disease.
4 more elements
A method of screening drugs for their effectiveness for treating or preventing a disease comprising:
- treating primary cells or cell lines in six or more tissue culture plate wells or vessels with a drug of interest, wherein multiple primary cells or cell lines are treated, each in their own tissue culture plate well or vessel;
- optionally lysing the primary cells or cell lines;
- adding six or more reagents to the six or more tissue culture plate wells or vessels, wherein at least one reagent is added to each of the tissue culture plate wells or vessels;
- conducting an assay reaction in each well or vessel of said tissue culture plate, wherein each reagent and or assay is chosen to measure a particular node in the disease pathway for the disease for which drugs are being screened;
- and
- analyzing the effect the drug of interest has on each of the nodes of the disease pathway, thereby determining whether the drug of interest would be effective for treating or preventing the disease.
3 more elements
A kit for screening compounds to determine whether they are effective for treating or preventing a disorder comprising:
- one or more microarray plates;
- two or more cell lines and/or cell lysates;
- and
- six or more reagents.
1 more element
Dependencies are inferred from the claim text.