Method for stabilizing or preserving nucleic acid by using amino surfactants
AssigneeIndustrial Technology Research Institute
InventorsTung-Liang Huang, Shang-Chi Lin
Life of this patent
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Abstract
A method for stabilizing or persevering nucleic acids by forming an insoluble ionic complex between nucleic acids and a surfactant in a biological sample, consisting of a step of contacting the biological sample with an isolation reagent comprising amino surfactants of the formula (I): R1R2R3N(O)x, (I), wherein, R1 and R2 each independently is H, C1-C6 alkyl group, C6-C12 aryl group, or C6-C12 aralkyl group; R3 is C1-C20 alkyl group, C6-C26 aryl group or C6-C26 aralkyl group; and x is an integer of 0 or 1. Moreover, the concentration of the amino surfactants in the reagent ranges from 0.001% to 20%. The present invention also relates to a reagent for stabilizing or preserving nucleic acids in a biological sample.
Claims30
A method for stabilizing or preserving nucleic acids by forming an insoluble ionic complex between nucleic acids and a surfactant in a biological sample, comprising a step of contacting the biological sample with a stabilizing or preserving composition comprising amino surfactants of the formula (I):
- R1R2R3N(O)x, (I),
- wherein, R1 and R2 each independently is H, C1-C6 alkyl group, C6-C12 aryl group, or C6-C12 aralkyl group;
- R3 is C1-C20 alkyl group, C6-C26 aryl group or C6-C26 aralkyl group;
- and x is an integer of 0 or 1.
A reagent for stabilizing or preserving nucleic acids in a biological sample comprising amino surfactants of the formula (I):
- R1R2R3N(O)x, (I),
- wherein, R1 and R2 each independently is H, C1-C6 alkyl group, C6-C12 aryl group, or C6-C12 aralkyl group;
- R3 is C1-C20 alkyl group, C6-C26 aryl group or C6-C26 aralkyl group;
- and x is an integer of 0 or 1.
Dependencies are inferred from the claim text.