Stabilization of viral compositions
OwnerGary Calton, Rita Fishelevichinventors
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Abstract
This invention concerns stabilized virus compositions, preferably a herpesvirus which may be an attenuated or genetically modified herpes simplex virus or varicella zoster virus, and a method of stabilizing viruses and immunizing preparations by the addition of sugars, preferably glucose and amino acids, preferably lysine.
Claims21
A live virus preparation comprising:
- (a) herpes simplex and
- (b) lysine at a concentration of 0.2 to 200 g/l. and
- (c) a sugar or sugar alcohol at 0.2 to 200 g/l.
A stabilizing agent for live herpes virus viral immunizing agent comprising lysine and glucose.
A method of preparing a live virus immunizing agent that comprises mixing a live herpes virus with a stabilizer comprising an aqueous solution of a sugar or sugar alcohol at 1-100 g/l and lysine at 2 to 20 g/l.
A method of harvesting live herpes virus for use in a live virus immunizing agent that includes the step of disrupting cells containing said virus in the presence of a stabilizer comprising an aqueous solution of a sugar or sugar alcohol at 1-200 g/l and lysine at 0.2 to 200 g/l.
A method of harvesting herpes simplex virus for use in a live virus immunizing agent that includes the step of separating the growth medium from the cells in which the herpes simplex is grown and adding a stabilizer to the growth medium containing excreted virus comprising lysine and sugar or sugar alcohol to provide a final concentration of the sugar or sugar alcohol at 1-200 g/l and of the lysine at 0.2 to 200 g/l.
Dependencies are inferred from the claim text.