Biomolecule detecting element and method for analyzing nucleic acid using the same
OwnerYuji Miyahara, Toshiya Sakata +2inventors
InventorsYuji Miyahara, Toshiya Sakata, Masao Kamahori, Yoshiaki Yazawa
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Abstract
An inexpensive DNA chip/DNA microarray system capable of highly accurate measurement with low running cost. A DNA probe 8 is immobilized on a floating electrode 7 connected to a gate electrode 5 of a field effect transistor. Hybridization with a target gene is carried out on the surface of the floating electrode, when a change in surface charge density is detected using a field effect.
Claims4
1-10. (canceled)
A method for analyzing nucleic acids using an insulated gate field effect transistor on which a biomolecular probe is immobilized as a biomolecule detecting element, comprising the steps of:
- immobilizing a single-stranded nucleic acid probe on said biomolecule detecting element as said biomolecular probe;
- introducing a sample solution containing at least one kind of nucleic acid onto said biomolecule detecting element and carrying out hybridization with said single-stranded nucleic acid probe;
- introducing a washing solution onto said biomolecule detecting element and removing unreacted nucleic acid on said biomolecule detecting element;
- introducing an intercalator solution onto said biomolecule detecting element and causing it to react with the nucleic acid that has become double-stranded;
- introducing a washing solution onto said biomolecule detecting element and removing unreacted intercalator on said biomolecule detecting element;
- and
- introducing a buffer solution onto said biomolecule detecting element and measuring output values of said insulated gate field effect transistor.
A method for analyzing nucleic acids using a biomolecule detecting element, comprising an insulated gate field effect transistor on which a biomolecular probe is immobilized, a transmission/reception antenna, a reception circuit, and a transmission circuit, comprising the steps of:
- putting a plurality of biomolecule detecting elements having different kinds of single-stranded nucleic acid probes immobilized thereon as said biomolecular probe, and a buffer solution in a reaction vessel, and receiving a signal from each of said biomolecule detecting elements using an external receiver;
- introducing a sample solution containing at least one kind of nucleic acid into said reaction vessel and carrying out hybridization with said single-stranded nucleic acid probe;
- introducing an intercalator solution into said reaction vessel and causing it to react with the nucleic acid that has become double-stranded;
- and
- receiving a signal from each of said biomolecule detecting elements using an external receiver.
Dependencies are inferred from the claim text.