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US 2006/0141467 A1Published application
Methods and kits for making double stranded ribonucleic acids
AssigneeQ-RNA, Inc.
InventorBrian Zeiler
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Abstract
Embodiments of the present invention are directed to methods and kits for the generation of double stranded RNA by RNA dependent RNA polymerases. The methods and kits feature exponential generation of such RNAs through simple steps/ The RNA is suitable for interference with expression in cells.
Claims26
A method of making double stranded RNA having a selected sequence comprising the steps of:
- a) forming an admixture of an RNA dependent RNA polymerase, reagents for the synthesis of transcript nucleic acids, and at least one template nucleic acid, said template nucleic acid acting as a template for the synthesis of RNA encoding said selected sequence upon the imposition of nucleic acid synthesis conditions and in the presence of said reagents and RNA dependent RNA polymerase;
- b) imposing nucleic acid synthesis conditions on said admixture to form an amplification product comprising double stranded RNA encoding the selected sequence.
A kit for making double stranded RNA comprising:
- a) an RNA dependent RNA polymerase which RNA dependent RNA polymerase synthesizes double stranded nucleic acid in the presence of reagents;
- b) reagents for the synthesis of transcript nucleic acids;
- c) means for making at least one template nucleic acid, said template nucleic acid acting as a template for the synthesis of RNA encoding said selected sequence upon the imposition of nucleic acid synthesis conditions and in the presence of said reagents and RNA dependent RNA polymerase;
- d) instructions for imposing nucleic acid synthesis conditions on said admixture to form an amplification product comprising double stranded RNA encoding the selected sequence.
1 more element
Dependencies are inferred from the claim text.