Methods and compositions for milieu-dependent binding of a targeted agent to a target
OwnerCynthia Edwards, Judith Fox +3inventors
InventorsCynthia Edwards, Judith Fox, Christopher Murray, Volker Schellenberger, Robert Tressler
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Abstract
The present invention provides methods and compositions for milieu-dependent binding of a targeted agent to a target, for example, for the milieu-dependent binding of a diagnostic or therapeutic molecule to a diseased, injured or infected organ, tissue or cell.
Claims33
A method of identifying a milieu-dependent targeted agent (MDTA), comprising contacting a microtarget in a first milieu and the microtarget in a second milieu with an agent, wherein the agent is a MDTA if it preferentially binds the microtarget in the first milieu over binding the microtarget in the second milieu.
A method of increasing the milieu-dependent binding of an agent, comprising
- a) contacting a microtarget in a first milieu with a modified form of the agent, wherein an unmodified form of the agent preferentially binds the microtarget in the first milieu relative to binding the microtarget in a second milieu, and
- b) selecting the modified form of the agent if its preference for binding the microtarget in the first milieu relative to its binding of the microtarget in the second milieu is greater than the preference of the unmodifed form of the agent for binding the microtarget in the first milieu relative to its binding the microtarget in the second milieu,
- wherein the selected agent is a milieu-dependent targeted agent (MDTA).
A method of preferentially binding a milieu-dependent targeted agent (MDTA) to a target comprising contacting the target and a non-target with the MDTA, wherein the target comprises a microtarget in a first milieu, the nontarget comprises the microtarget in a second milieu, and the first milieu is not identical to the second milieu, under conditions that allow the MDTA to preferentially bind the microtarget in the first milieu over binding the microtarget in the second milieu.
A method of binding a MDTA to at least one microtarget in a compartment, comprising manipulating the compartment and contacting the at least one microtarget with the MDTA under conditions that allow the at least one microtarget to bind the MDTA.
A method of detecting a diseased, injured or infected tissue from a subject comprising
- manipulating a compartment having the tissue
- contacting the tissue from the subject with a detectable MDTA that preferentially binds the diseased, injured or infected tissue over a healthy tissue, and
- detecting the binding of the detectable MDTA to the tissue from the subject,
- wherein an increase in binding of the detectable MDTA to the tissue from the subject relative to the binding of the MDTA to healthy tissue indicates that the subject has a diseased, injured or infected tissue.
A method of identifying a MDTA comprising
- a) manipulating a compartment, the compartment containing at least one microtarget and having a first milieu, the manipulation creating a second milieu in the compartment, the second milieu being different from the first milieu
- b) contacting the at least one microtarget in the first milieu with a modified form of an agent, wherein an unmodified form of the agent binds the at least one microtarget in the first milieu and the microtarget in the second milieu about equally, and
- c) selecting the modified form of the agent if it preferentially binds the at least one microtarget in the first milieu relative to its binding of the at least one microtarget in the second milieu,
- wherein the selected modified form of the agent is the MDTA.
A MDTA, comprising a binding moiety that preferentially binds to a microtarget present on a target relative to binding of the microtarget present on a non-target.
Dependencies are inferred from the claim text.