Prokineticin polypeptides, related compositions and methods
OwnerQun-Yong Zhou, Frederick Ehlertinventors
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Abstract
The invention provides isolated polypeptides that stimulate gastroeintestinal smooth muscle contraction, including human prokineticin 1 and human prokineticin 2 polypeptides, and functional fragments and modifications thereof. Also provided are methods of stimulating gastrointestinal smooth muscle contraction in a mammal, by administering to the mammal an effective amount of a prokineticin polypeptide. The invention also provides nucleic acid molecules encoding a prokineticin polypeptide, and antibodies that selectively bind a prokineticin polypeptide. Further provided are methods of identifying a prokineticin receptor ligand, agonist or antagonist.
Claims39
1-52. (canceled)
An isolated prokineticin receptor antagonist, comprising an amino acid sequence at least 80% identical to amino acids 7 to 77 of SEQ ID NO:3, said sequence comprising;
- (a) the 10 conserved cysteine residues of SEQ ID NO:3, and
- (b) from 0 to 9 of C-terminal amino acids 78 to 86 of SEQ ID NO:3, wherein amino acids 1 to 6 of said antagonist do not consist of amino acids AVITGA (SEQ ID NO:21).
An isolated prokineticin receptor antagonist, comprising an amino acid sequence at least 80% identical to amino acids 7 to 77 of SEQ ID NO:6, said sequence comprising;
- (a) the 10 conserved cysteine residues of SEQ ID NO:6, and
- (b) from 0 to 4 of C-terminal amino acids 78 to 81 of SEQ ID NO:6,
- wherein amino acids 1 to 6 of said antagonist do not consist of amino acids AVITGA (SEQ ID NO:21).
Dependencies are inferred from the claim text.