Tuberculosis nucleic acids, polypeptides and immunogenic compositions
AssigneeGene Therapy Systems, Inc.
InventorsDavid Roth, Huaping He
Life of this patent
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Abstract
The present invention provides transcriptionally active Mtb polynucleotides, recombinant Mtb peptides and polypeptides, and immunogenic Mtb antigens. Immunogenic compositions are also provided that may be useful as recombinant, subunit and DNA vaccines. In addition the invention provides diagnostic kits for Mtb.
Claims38
An isolated polynucleotide encoding a Mtb polypeptide that is antigenic in a mammal, wherein the polynucleotide is selected from the group consisting of:
- (a) SEQ ID NOS:
- 46-64, 110-121;
- or
- (b) a fragment thereof, wherein the fragment encodes an antigenic peptide epitope.
An isolated polynucleotide encoding an immunogenic Mtb antigen, wherein the polynucleotide is selected from the group consisting of:
- (a) SEQ ID NOS:
- 46-64, 110-121;
- or
- (b) a fragment thereof, wherein the fragment encodes an immunogenic peptide epitope.
A TAP polynucleotide comprising:
- (a) a 5′ TAP polynucleotide sequence;
- (b) a Mtb polynucleotide sequence;
- and
- (c) a 3′ TAP polynucleotide sequence.
A primer pair for amplifying a Mtb polynucleotide selected from the group consisting of:
- SEQ ID NOS:
- 8 and 9;
- 10 and 11;
- 12 and 13;
- 14 and 15;
- 16 and 17;
- 18 and 19;
- 20 and 21;
- 22 and 23;
- 24 and 25;
- 26 and 27;
- 28 and 29;
- 30 and 31;
- 32 and 33;
- 34 and 35;
- 36 and 37;
- 38 and 39;
- 40 and 41;
- 42 and 43;
- 44 and 45;
- 86 and 87;
- 88 and 89;
- 90 and 91;
- 92 and 93;
- 94 and 95;
- 96 and 97;
- 98 and 99;
- 100 and 101;
- 102 and 103;
- 104 and 105;
- 106 and,107;
- 108 and 109.
An isolated Mtb peptide or polypeptide that is antigenic, wherein said peptide or polypeptide is selected from:
- (a) SEQ ID NOS:
- 65-83, 122-133;
- or
- (b) a fragment thereof, wherein the fragment is antigenic.
An isolated Mtb peptide or polypeptide that is highly immunogenic, wherein the peptide or polypeptide is selected from the group consisting of:
- (a) SEQ ID NOS:
- 65-83, 122-133;
- or
- (b) a fragment thereof, wherein the fragment is immunogenic.
A recombinant Mtb peptide or polypeptide, wherein the amino terminus of said peptide or polypeptide comprises a HA tag or a His tag, and the carboxy terminus of said peptide or polypeptide is selected from the group consisting of:
- SEQ ID NOS:
- 65-83, 122-133.
A recombinant Mtb peptide or polypeptide, wherein the carboxy terminus of said peptide or polypeptide comprises a HA tag or a His tag, and the amino terminus of said peptide or polypeptide is selected from the group consisting of:
- SEQ ID NOS:
- 65-83, 122-133.
A recombinant Mtb peptide or polypeptide selected from the group consisting of:
- SEQ ID NOS:
- 65-83, 122-133, wherein said peptide or polypeptide is expressed in an in vitro transcription and translation system.
An immunogenic composition for inducing an immunological response in a mammalian host against Mtb, comprising a nucleic acid that encodes and expresses in vivo in a mammalian host cell at least one immunogenic peptide or polypeptide selected from the group consisting of:
- (a) SEQ ID NOS:
- 65-83, 122-133;
- or
- (b) a fragment thereof, wherein the fragment is immunogenic.
An immunogenic composition comprising at least one isolated Mtb peptide or polypeptide selected from the group consisting of:
- (a) SEQ ID NOS:
- 65-83, 122-133;
- or
- (b) a fragment thereof, wherein the fragment is immunogenic.
A method of generating an immune response in a mammalian host against Mtb comprising:
- administering to said mammalian host an immunogenic composition comprising at least one nucleic acid selected from the group of SEQ ID NO:
- 46-64, 110-121, fragments thereof or combinations thereof, wherein said nucleic acid encodes and expresses in vivo at least one immunogenic peptide or polypeptide, whereby said immune response against Mtb is generated.
A method of generating an immune response in a mammalian host against Mtb comprising:
- administering to said mammalian host an immunogenic composition comprising at least one nucleic acid encoding and expressing in vivo at least one immunogenic peptide or polypeptide selected from the group of SEQ ID NO:
- 65-83,122-133, fragments thereof or combinations thereof, whereby said immune response against Mtb is generated.
A method of generating an immune response in a mammalian host against Mtb comprising administering to said mammalian host an immunogenic composition comprising at least one immunogenic peptide or polypeptide selected from SEQ ID NO:
- 65-83, 122-133, fragment thereof or combinations thereof, whereby said immune response against Mtb is generated.
A kit comprising:
- a) at least one Mtb immunogenic composition selected from:
- i) a nucleic acid selected from the group consisting of SEQ ID NO:
- 46-64, 110-121, fragments thereof or combinations thereof;
- or
- ii) a peptide selected from the group consisting of SEQ ID NO:
- 65-83, 122-133, fragments thereof or combinations thereof;
- and
- b) an adjuvant.
Dependencies are inferred from the claim text.