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US 2006/0140975 A1Published application
Regulated bacterial lysis for gene vaccine vector delivery and antigen release
OwnerRoy Curtiss, Wei Konginventors
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Abstract
The present invention generally relates to regulated host-vector systems for delivery of genetic vaccine vector or desired gene products to a eukaryotic host, preparations of bacterial genetic vaccines, genetically engineered microorganisms that are useful for delivery of genetic vaccine vectors or desired gene products, and methods of use for the host-vector system.
Claims64
A host-vector system, which comprises
- a. A host chromosome comprising
- i. An activatible control sequence, wherein the activatible control sequence is activatible by an inducer;
- ii. a sequence that encodes a repressor, wherein the sequence is operably-linked to the activatible control sequence;
- and
- iii. at least one essential gene, wherein the essential gene encodes a polypeptide that is necessary for synthesis of a rigid layer of a cell wall of a prokaryote, and wherein the essential gene is inactivated;
- AND
- b. A vector comprising
- i. a eukaryotic expression cassette comprising
- 1. A eukaryotic promoter sequence;
- 2. A site for insertion of a gene encoding a desired gene product;
- and
- 3. A polyadenylation sequence;
- ii. a prokaryotic activator-promoter sequence;
- iii. at least one origin of replication (ori);
- iv. a regulatable prokaryotic promoter, which is repressible by the repressor;
- v. at least one essential gene, wherein the essential gene is necessary for synthesis of a rigid layer of a cell wall of a prokaryote;
- vi. at least one transcription terminator sequence;
- and
- vii. at least one CpG sequence motif, wherein the CpG sequence motif enhances immunogenicity.
16 more elements
A host-vector system, which comprises
- a. A host chromosome comprising
- i. An activatible control sequence, wherein the activatible control sequence is activatible by an inducer;
- ii. at least one sequence that encodes a repressor, wherein the sequence is operably-linked to the activatible control sequence;
- and
- iii. at least one essential gene, wherein the essential gene encodes a polypeptide that is necessary for synthesis of a rigid layer of a cell wall of a prokaryote, and wherein the essential gene is operably linked to the activatible control sequence;
- AND
- b. A vector comprising
- i. a eukaryotic expression cassette comprising
- 1. a eukaryotic promoter sequence;
- 2. a site for insertion of a gene encoding a desired gene product;
- and
- 3. a polyadenylation sequence;
- ii. a prokaryotic activator-promoter sequence;
- iii. at least one origin of replication (ori);
- iv. a regulatable prokaryotic promotor sequence, wherein the regulatable prokaryotic promotor sequence is repressible by the repressor;
- v. at least one essential gene, wherein the essential gene is necessary for synthesis of a rigid layer of a cell wall of a prokaryote;
- vi. at least one transcription terminator sequence;
- and
- vii. at least one CpG sequence motif, wherein the CpG sequence motif enhances immunogenicity.
16 more elements
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A host-vector system, which comprises
- a. A host chromosome comprising
- i. an activatible control sequence, wherein the activatible control sequence is activatible by an inducer;
- ii. at least one sequence that encodes a repressor, wherein the sequence is operably-linked to the activatible control sequence;
- and
- iii. at least one essential gene, wherein the essential gene encodes a polypeptide that is necessary for synthesis of a rigid layer of a cell wall of a prokaryote, and wherein the essential gene is inactivated;
- AND
- b. At least one vector comprising
- i. a prokaryotic activator-promoter sequence;
- ii. at least one origin of replication (ori);
- iii. a first regulatable prokaryotic promotor sequence, wherein the first regulatable prokaryotic promotor sequence is repressible by a first repressor;
- iv. a second regulatable prokaryotic promotor sequence, wherein the second regulatable prokaryotic promotor sequence is repressible by a second repressor;
- v. at least one essential gene, wherein the essential gene is necessary for synthesis of a rigid layer of a cell wall of a prokaryote;
- vi. at least one transcription terminator sequence;
- and
- vii. a site for insertion of a gene encoding a desired gene product.
12 more elements
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Dependencies are inferred from the claim text.