Human il-1 beta antagonists
AssigneeApplied Molecular Evolution, Inc.
InventorsCraig Dickinson, Alain Vasserot, Jeffry Watkins, Jirong Lu
Life of this patent
Estimating the term…
Abstract
The present invention encompasses isolated antibodies, or antigen-binding portions thereof, that specifically bind mature human IL-1 Beta. These antibodies, or antigen-binding portions thereof, generally exhibit high binding affinities (low kooff values), reduced deamidation compared to the native antibody, and can be used to treat various diseases such as rheumatoid arthritis, osteoarthritis, or neuroinflammation.
Claims16
1-137. (canceled)
A humanized antibody, or an antigen-binding portion thereof, that specifically binds human IL-1β, selected from the group consisting of:
- (a) a light chain variable region comprising an amino acid sequence encoded by the polynucleotide sequence of SEQ ID NO:60 and a heavy chain variable region comprising an amino acid sequence of SEQ ID NO:55;
- (b) a light chain variable region comprising an amino acid sequence encoded by the polynucleotide sequence of SEQ In NO:61 and a heavy chain variable region comprising an amino acid sequence of SEQ ED NO:56;
- (c) a light chain variable region comprising an amino acid sequence encoded by the polynucleotide sequence of SEQ ID NO:62 and a heavy chain variable region comprising an amino acid sequence of SEQ ID NO:57;
- (d) a light chain variable region comprising an amino acid sequence encoded by the polynucleotide sequence of SEQ ID NO:63 and a heavy chain variable region comprising an amino acid sequence of SEQ ID NO:58;
- and,
- (e) a light chain variable region comprising an amino acid sequence encoded by the polynucleotide sequence of SEQ ID NO:64 and a heavy chain variable region comprising an amino acid sequence of SEQ ID NO-59;
A humanized antibody, or an antigen-binding portion thereof, that specifically binds human IL-1β, comprising a light chain and a heavy chain selected from the group consisting of:
- (a) a light chain comprising an amino acid sequence of SEQ ID NO:46 and a heavy chain comprising an amino acid sequence of SEQ ID NO:
- 45;
- (b) a light chain comprising an amino acid sequence of SEQ ID NO:47 and a heavy chain comprising an amino acid sequence of SEQ ID NO:
- 48
- (c) a light chain comprising an amino acid sequence of SEQ ID NO:48 and a heavy chain comprising an amino acid sequence of SEQ ID NO:68;
- (d) a light chain comprising an amino acid sequence of SEQ K) NO:49 and a heavy chain comprising an amino acid sequence of SEQ ID NO:50;
- (e) a light chain comprising an amino acid sequence of SEQ ID NO:51 and a heavy chain comprising an amino acid sequence of SEQ ID NO:
- 52;
- and,
- (f) a light chain comprising an amino acid sequence of SEQ ID NO:53 and a heavy chain comprising an amino acid sequence of SEQ ID NO:54.
A humanized antibody, or an antigen-binding portion thereof, that specifically binds human IL-1β, wherein said antibody has a light chain of SEQ ID NO:
- 48 and a heavy chain of SEQ ID NO:68.
Dependencies are inferred from the claim text.