Bioartificial device for propagation of tissue, preparation and uses thereof

A method for the preparation of a bioartificial composite useful for repair of tissues or organs in a host, said method comprising:

• a. preparing a substrate having a surface having the morphological characteristics of the desired tissues or organs said surface permitting the reception and growth promoting retention of a cellular preparation, wherein the cellular preparation comprises a quantity of cells capable of growth and aggregation to form a component of said tissues or organs;
• b. applying the cellular preparation to the surface of said substrate to form a first layer of adherent cells disposed on said surface having a pattern of morphological orientation aligned uniformly, wherein said first layer acts as a template to accept a second layer of cells upon said first layer, said second layer comprising an organized layer oriented in the direction of said first layer;
• and
• c. implanting the bioartificial composite of Step b. at the location of desired repair, whereby the growth of said tissue takes place in the host.

A method for the preparation of tissue useful for repair of tissues or organs in a host, said method comprising:

• a. preparing a substrate having a surface having the morphological characteristics of the desired tissue, said surface permitting the reception and growth promoting retention of a cellular preparation, wherein the cellular preparation comprises a quantity of cells capable of growth and aggregation to form said tissue;
• b. applying the cellular preparation to the surface of Step a to form a first layer of adherent cells disposed on said surface having a pattern of morphological orientation aligned uniformly, wherein said first layer acts as a template to accept a second layer of cells upon said first layer, said second layer comprising an organized layer oriented in the direction of said first layer;
• c. incubating the substrate of Step b. under conditions promoting the growth of said tissue thereon;
• and
• d. recovering the tissue prepared in Step c.

A method for the preparation of tissue useful for testing, development and discovery, said method comprising:

• a. preparing a substrate defining a surface having the following characteristics:
• i. at least one cell accepting surface defined by an oriented surface roughness of at least 200 nm root mean squared;
• ii. at least one cell accepting surface defined by a surface curvature of equal or greater than 0.016 microns−1;
• and
• iii. said substrate defines a repeating surface structure that enables a pattern of morphological orientation of cells applied thereto to become aligned uniformly;
• b. applying to the surface of Step a. a cellular preparation, said cellular preparation comprising a quantity of cells capable of growth and aggregation to form a first layer of cells having uniformly-aligned morphological orientation, the first layer being suitable to act as a template to accept a second layer of cells thereon and orient the second layer of cells in the direction of the first layer;
• c. incubating the bioartificial product of Step b. with a different type of cell to effect growth of said tissue thereon;
• and
• d. recovering the tissue prepared in Step c.;
• wherein said tissue may be used as a benchtop testing system or tissue surrogate.

A method for the preparation of tissue useful for repair of tissues or organs in a host, said method comprising:

• a. preparing a substrate defining a surface having the following characteristics:
• i. at least one cell accepting surface defined by an oriented surface roughness of at least 200 nm root mean squared;
• ii. at least one cell accepting surface defined by a surface curvature of equal or greater than 0.016 microns−1;
• and
• iii. said substrate defines a repeating surface structure that enables a pattern of morphological orientation of cells applied thereto to become aligned uniformly;
• b. applying to the surface of Step a. a cellular preparation, said cellular preparation comprising a quantity of cells capable of growth and aggregation to form a first layer of cells having uniformly-aligned morphological orientation, the first layer being suitable to act as a template to accept a second layer of cells thereon and orient the second layer of cells in the direction of the first layer;
• c. incubating the bioartificial product of Step b. with a different type of cell to affect growth of said tissue thereon;
• and d. recovering the tissue prepared in Step c;
• wherein said tissue may be used for therapeutic purposes.