Assay of hair proteins
AssigneeL'oreal
InventorsMarine Peron, Arnaud Lagarde, Joël Bover
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Abstract
A process for detecting previous treatment of keratinous fibers with alkaline technology for hair straightening or smoothing includes: bringing the keratinous fibers into contact with an extraction solution under conditions effective to cause dissolution of keratin proteins; and conducting a qualitative and/or quantitative assay of the keratin proteins dissolved in the extraction solution. The extraction solution may include a mixture of at least one of urea, thiourea and derivatives thereof with at least one reducing agent, and the at least one reducing agent may be free from thiourea. A kit for performing such a process is also provided.
Claims27
A process for detecting previous treatment of keratinous fibers with alkaline technology for hair straightening or smoothing, the process comprising:
- bringing the keratinous fibers into contact with an extraction solution under conditions effective to cause dissolution of keratin proteins;
- and
- conducting a qualitative and/or quantitative assay of the keratin proteins dissolved in the extraction solution;
- wherein:
- the extraction solution comprises a mixture of at least one of urea, thiourea and derivatives thereof, with at least one reducing agent;
- and
- the at least one reducing agent is free from thiourea.
A kit for characterizing a protein state of keratinous fibers, comprising:
- a solution comprising at least one of urea, thiourea, and derivatives thereof;
- at least one reducing agent, the reducing agent being free from thiourea;
- and
- at least one compound capable of functioning as a colorimetric indicator for qualitatively and/or quantitatively assaying extracted keratin proteins.
Dependencies are inferred from the claim text.