Use of high wavenumber raman spectroscopy for measuring tissue

An instrument for measuring a Raman signal of tissue, the instrument comprising a laser, a signal detection unit for measuring the Raman signal, and a fiber optic probe, wherein the fiber optic probe comprises one or more optical fibers for directing laser light onto the tissue and for collecting light that is scattered by the tissue and guiding the collected light away from the tissue towards the signal detection unit, the fiber or fibers comprising a core, a cladding and optionally a coating, and the fiber or fibers for collecting light having substantially no Raman signal in one or more parts of the 2500-3700 cm−1 spectral region, and wherein the detection unit records the Raman signal scattered by the tissue in said spectral region, the instrument further comprising a signal analysis unit which analyses the recorded Raman signal in one or more parts of the 2500-3700 cm−1 spectral region, the analysis comprising an algorithm which outputs data regarding the molecular composition of the tissue and/or the clinical diagnostic class to which the tissue belongs.

Method of using of an instrument for measuring a Raman signal of tissue, the instrument comprising a laser, a signal detection unit for measuring the Raman signal, and a fiber optic probe, wherein the fiber optic probe comprises one or more optical fibers for directing laser light onto the tissue and for collecting light that is scattered by the tissue and guiding the collected light away from the tissue towards the signal detection unit, the fiber or fibers comprising a core, a cladding and optionally a coating, and the fiber or fibers for collecting light having substantially no Raman signal in one or more parts of the 2500-3700 cm−1 spectral region, and wherein the detection unit records the Raman signal scattered by the tissue in said spectral region, the instrument further comprising a signal analysis unit which analyses the recorded Raman signal in one or more parts of the 2500-3700 cm−1 spectral region, the analysis comprising an algorithm which outputs data regarding the molecular composition of the tissue and/or the clinical diagnostic class to which the tissue belongs.

A method for producing and measuring a Raman signal of tissue, comprising providing a laser, a detection unit for measuring a Raman signal, and a fiber optic probe, wherein the fiber optic probe comprises one or more optical fibers for directing laser light onto the tissue and for collecting light that is scattered by the tissue and guiding the collected light away from the tissue toward the signal detection unit, the fiber or fibers comprising a core, a cladding and optionally a coating, sending laser light through the one or more optical fibers, receiving the Raman signal from the tissue through the one or more optical fibers and detecting the Raman signal by a signal detection unit, the fiber or fibers for collecting light having substantially no Raman signal in one or more parts of the 2500-3700 cm−1 spectral region, and wherein the signal detection unit records the Raman signal in said spectral region, the instrument further comprising a signal analysis unit which analyses the recorded Raman signal in one or more parts of the 2500-3700 cm−1 spectral region, the analysis comprising an algorithm which outputs data regarding the molecular composition of the tissue and/or the clinical diagnostic class to which the tissue belongs.

Method for evaluating an optical fiber for measuring a Raman signal of tissue, wherein an instrument according to claim 1 is used and wherein a tissue sample is excised, biopted or taken from a human or animal body before measuring, and wherein the Raman signal of the optical fiber is measured of the sample and of a blanc, and wherein the Raman signals of the sample and of the blanc are compared.