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US 2006/0138320 A1Published application
Mass spectrometer
AssigneeMicromass UK Limited
InventorRobert Bateman
Life of this patent
Estimating the term…
Abstract
A method of mass spectrometry is disclosed wherein a collision, fragmentation or reaction device is repeatedly switched between a high fragmentation or reaction mode and a low fragmentation or reaction mode. Parent ions from a first sample are passed through the device and parent ion mass spectra and fragmentation ion mass spectra are obtained. Parent ions from a second sample are then passed through the device and a second set of parent ion mass spectra and fragmentation ion mass spectra are obtained. The mass spectra are then compared and if either certain parent ions or certain fragmentation ions in the two samples are expressed differently then further analysis is performed to seek to identify the ions which are expressed differently in the two different samples.
Claims54
A method of mass spectrometry comprising:
- passing parent or precursor ions from a first sample to a collision, fragmentation or reaction device;
- repeatedly switching, altering or varying said collision, fragmentation or reaction device between a first mode wherein at least some of said parent or precursor ions from said first sample are fragmented or reacted to form one or more fragment, product, daughter or adduct ions and a second mode wherein substantially fewer parent or precursor ions are fragmented or reacted;
- passing parent or precursor ions from a second sample to a collision, fragmentation or reaction device;
- repeatedly switching, altering or varying said collision, fragmentation or reaction device between a first mode wherein at least some of said parent or precursor ions from said second sample are fragmented or reacted to form one or more fragment, product, daughter or adduct ions and a second mode wherein substantially fewer parent or precursor ions are fragmented or reacted;
- automatically determining the intensity of first parent or precursor ions from said first sample which have a first mass to charge ratio;
- automatically determining the intensity of second parent or precursor ions from said second sample which have said same first mass to charge ratio;
- and
- comparing the intensity of said first parent or precursor ions with the intensity of said second parent or precursor ions;
- wherein if the intensity of said first parent or precursor ions differs from the intensity of said second parent or precursor ions by more than a predetermined amount then either said first parent or precursor ions and/or said second parent or precursor ions are considered to be parent or precursor ions of interest;
- and
- wherein said collision, fragmentation or reaction device is selected from the group consisting of:
- (i) a Surface Induced Dissociation (“SID”) fragmentation device;
- (ii) an Electron Transfer Dissociation fragmentation device;
- (iii) an Electron Capture Dissociation fragmentation device;
- (iv) an Electron Collision or Impact Dissociation fragmentation device;
- (v) a Photo Induced Dissociation (“PID”) fragmentation device;
- (vi) a Laser Induced Dissociation fragmentation device;
- (vii) an infrared radiation induced dissociation device;
- (viii) an ultraviolet radiation induced dissociation device;
- (ix) a nozzle-skimmer interface fragmentation device;
- (x) an in-source fragmentation device;
- (xi) an ion-source Collision Induced Dissociation fragmentation device;
- (xii) a thermal or temperature source fragmentation device;
- (xiii) an electric field induced fragmentation device;
- (xiv) a magnetic field induced fragmentation device;
- (xv) an enzyme digestion or enzyme degradation fragmentation device;
- (xvi) an ion-ion reaction fragmentation device;
- (xvii) an ion-molecule reaction fragmentation device;
- (xviii) an ion-atom reaction fragmentation device;
- (xix) an ion-metastable ion reaction fragmentation device;
- (xx) an ion-metastable molecule reaction fragmentation device;
- (xxi) an ion-metastable atom reaction fragmentation device;
- (xxii) an ion-ion reaction device for reacting ions to form adduct or product ions;
- (xxiii) an ion-molecule reaction device for reacting ions to form adduct or product ions;
- (xxiv) an ion-atom reaction device for reacting ions to form adduct or product ions;
- (xxv) an ion-metastable ion reaction device for reacting ions to form adduct or product ions;
- (xxvi) an ion-metastable molecule reaction device for reacting ions to form adduct or product ions;
- and (xxvii) an ion-metastable atom reaction device for reacting ions to form adduct or product ions.
35 more elements
A method of mass spectrometry comprising:
- passing parent or precursor ions from a first sample to a collision, fragmentation or reaction device;
- repeatedly switching, altering or varying said collision, fragmentation or reaction device between a first mode wherein at least some of said parent or precursor ions from said first sample are fragmented or reacted to form one or more fragment, product, daughter or adduct ions and a second mode wherein substantially fewer parent or precursor ions are fragmented or reacted;
- passing parent or precursor ions from a second sample to a collision, fragmentation or reaction device;
- repeatedly switching, altering or varying said collision, fragmentation or reaction device between a first mode wherein at least some of said parent or precursor ions from said second sample are fragmented or reacted to form one or more fragment, product, daughter or adduct ions and a second mode wherein substantially fewer parent or precursor ions are fragmented or reacted;
- automatically determining the intensity of first parent or precursor ions from said first sample which have a first mass to charge ratio;
- automatically determining the intensity of second parent or precursor ions from said second sample which have said same first mass to charge ratio;
- determining a first ratio of the intensity of said first parent or precursor ions to the intensity of other parent or precursor ions in said first sample;
- determining a second ratio of the intensity of said second parent or precursor ions to the intensity of other parent or precursor ions in said second sample;
- and
- comparing said first ratio with said second ratio;
- wherein if said first ratio differs from said second ratio by more than a predetermined amount then either said first parent or precursor ions and/or said second parent or precursor ions are considered to be parent or precursor ions of interest;
- and
- wherein said collision, fragmentation or reaction device is selected from the group consisting of:
- (i) a Surface Induced Dissociation (“SID”) fragmentation device;
- (ii) an Electron Transfer Dissociation fragmentation device;
- (iii) an Electron Capture Dissociation fragmentation device;
- (iv) an Electron Collision or Impact Dissociation fragmentation device;
- (v) a Photo Induced Dissociation (“PID”) fragmentation device;
- (vi) a Laser Induced Dissociation fragmentation device;
- (vii) an infrared radiation induced dissociation device;
- (viii) an ultraviolet radiation induced dissociation device;
- (ix) a nozzle-skimmer interface fragmentation device;
- (x) an in-source fragmentation device;
- (xi) an ion-source Collision Induced Dissociation fragmentation device;
- (xii) a thermal or temperature source fragmentation device;
- (xiii) an electric field induced fragmentation device;
- (xiv) a magnetic field induced fragmentation device;
- (xv) an enzyme digestion or enzyme degradation fragmentation device;
- (xvi) an ion-ion reaction fragmentation device;
- (xvii) an ion-molecule reaction fragmentation device;
- (xviii) an ion-atom reaction fragmentation device;
- (xix) an ion-metastable ion reaction fragmentation device;
- (xx) an ion-metastable molecule reaction fragmentation device;
- (xxi) an ion-metastable atom reaction fragmentation device;
- (xxii) an ion-ion reaction device for reacting ions to form adduct or product ions;
- (xxiii) an ion-molecule reaction device for reacting ions to form adduct or product ions;
- (xxiv) an ion-atom reaction device for reacting ions to form adduct or product ions;
- (xxv) an ion-metastable ion reaction device for reacting ions to form adduct or product ions;
- (xxvi) an ion-metastable molecule reaction device for reacting ions to form adduct or product ions;
- and (xxvii) an ion-metastable atom reaction device for reacting ions to form adduct or product ions.
37 more elements
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7-13. (canceled)
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A method as claimed in claim 4815, wherein the step of identifying parent or precursor ions of interest comprises determining whether said parent or precursor ions of interest are observed in a mass spectrum obtained when said collision, fragmentation or reaction device is in said second mode for a certain time period and said fragment, product, daughter or adduct ions are observed in a mass spectrum obtained either immediately before said certain time period, when said collision, fragmentation or reaction device is in said first mode, or immediately after said certain time period, when said collision, fragmentation or reaction device is in said first mode.
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A mass spectrometer comprising:
- a collision, fragmentation or reaction device which is arranged and adapted to be repeatedly switched, altered or varied in use between a first mode wherein at least some parent or precursor ions are fragmented or reacted into one or more fragment, product, daughter or adduct ions and a second mode wherein substantially fewer parent or precursor ions are fragmented or reacted;
- a mass analyser;
- and
- a control system which in use:
- (i) determines the intensity of first parent or precursor ions from a first sample which have a first mass to charge ratio;
- (ii) determines the intensity of second parent or precursor ions from a second sample which have said same first mass to charge ratio;
- and
- (iii) compares the intensity of said first parent or precursor ions with the intensity of said second parent or precursor ions;
- wherein if the intensity of said first parent or precursor ions differs from the intensity of said second parent or precursor ions by more than a predetermined amount then either said first parent or precursor ions and/or said second parent or precursor ions are considered to be parent or precursor ions of interest;
- wherein said collision, fragmentation or reaction device is selected from the group consisting of:
- (i) a Surface Induced Dissociation (“SID”) fragmentation device;
- (ii) an Electron Transfer Dissociation fragmentation device;
- (iii) an Electron Capture Dissociation fragmentation device;
- (iv) an Electron Collision or Impact Dissociation fragmentation device;
- (v) a Photo Induced Dissociation (“PID”) fragmentation device;
- (vi) a Laser Induced Dissociation fragmentation device;
- (vii) an infrared radiation induced dissociation device;
- (viii) an ultraviolet radiation induced dissociation device;
- (ix) a nozzle-skimmer interface fragmentation device;
- (x) an in-source fragmentation device;
- (xi) an ion-source Collision Induced Dissociation fragmentation device;
- (xii) a thermal or temperature source fragmentation device;
- (xiii) an electric field induced fragmentation device;
- (xiv) a magnetic field induced fragmentation device;
- (xv) an enzyme digestion or enzyme degradation fragmentation device;
- (xvi) an ion-ion reaction fragmentation device;
- (xvii) an ion-molecule reaction fragmentation device;
- (xviii) an ion-atom reaction fragmentation device;
- (xix) an ion-metastable ion reaction fragmentation device;
- (xx) an ion-metastable molecule reaction fragmentation device;
- (xxi) an ion-metastable atom reaction fragmentation device;
- (xxii) an ion-ion reaction device for reacting ions to form adduct or product ions;
- (xxiii) an ion-molecule reaction device for reacting ions to form adduct or product ions;
- (xxiv) an ion-atom reaction device for reacting ions to form adduct or product ions;
- (xxv) an ion-metastable ion reaction device for reacting ions to form adduct or product ions;
- (xxvi) an ion-metastable molecule reaction device for reacting ions to form adduct or product ions;
- and (xxvii) an ion-metastable atom reaction device for reacting ions to form adduct or product ions.
34 more elements
A mass spectrometer comprising:
- a collision, fragmentation or reaction device which is arranged and adapted to be repeatedly switched, altered or varied in use between a first mode wherein at least some parent or precursor ions are fragmented or reacted into one or more fragment, product, daughter or adduct ions and a second mode wherein substantially fewer parent or precursor ions are fragmented or reacted;
- a mass analyser;
- and
- a control system which in use:
- (i) determines the intensity of first parent or precursor ions from a first sample which have a first mass to charge ratio;
- (ii) determines the intensity of second parent or precursor ions from a second sample which have said same first mass to charge ratio;
- (iii) determines a first ratio of the intensity of said first parent or precursor ions to the intensity of other parent or precursor ions in said first sample;
- (iv) determines a second ratio of the intensity of said second parent or precursor ions to the intensity of other parent or precursor ions in said second sample;
- and
- (v) compares said first ratio with said second ratio;
- wherein if said first ratio differs from said second ratio by more than a predetermined amount then either said first parent or precursor ions and/or said second parent or precursor ions are considered to be parent or precursor ions of interest;
- wherein said collision, fragmentation or reaction device is selected from the group consisting of:
- (i) a Surface Induced Dissociation (“SID”) fragmentation device;
- (ii) an Electron Transfer Dissociation fragmentation device;
- (iii) an Electron Capture Dissociation fragmentation device;
- (iv) an Electron Collision or Impact Dissociation fragmentation device;
- (v) a Photo Induced Dissociation (“PID”) fragmentation device;
- (vi) a Laser Induced Dissociation fragmentation device;
- (vii) an infrared radiation induced dissociation device;
- (viii) an ultraviolet radiation induced dissociation device;
- (ix) a nozzle-skimmer interface fragmentation device;
- (x) an in-source fragmentation device;
- (xi) an ion-source Collision Induced Dissociation fragmentation device;
- (xii) a thermal or temperature source fragmentation device;
- (xiii) an electric field induced fragmentation device;
- (xiv) a magnetic field induced fragmentation device;
- (xv) an enzyme digestion or enzyme degradation fragmentation device;
- (xvi) an ion-ion reaction fragmentation device;
- (xvii) an ion-molecule reaction fragmentation device;
- (xviii) an ion-atom reaction fragmentation device;
- (xix) an ion-metastable ion reaction fragmentation device;
- (xx) an ion-metastable molecule reaction fragmentation device;
- (xxi) an ion-metastable atom reaction fragmentation device;
- (xxii) an ion-ion reaction device for reacting ions to form adduct or product ions;
- (xxiii) an ion-molecule reaction device for reacting ions to form adduct or product ions;
- (xxiv) an ion-atom reaction device for reacting ions to form adduct or product ions;
- (xxv) an ion-metastable ion reaction device for reacting ions to form adduct or product ions;
- (xxvi) an ion-metastable molecule reaction device for reacting ions to form adduct or product ions;
- and (xxvii) an ion-metastable atom reaction device for reacting ions to form adduct or product ions.
36 more elements
58-69. (canceled)
71-74. (canceled)
Dependencies are inferred from the claim text.