Method of controlling migration of substance
AssigneeKatayanagi Institute
InventorsKazuyoshi Yano, Isao Karube, Atsunori Hiratsuka, Shuo-Wen Tsai
Life of this patent
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Abstract
Transfer of fluid substances, and/or substances comprised in fluid substances, is controlled by introducing a separation medium, which prevents transfer of the fluid substances, and/or the substances comprised in the fluid substances, to an intermediate cavity connecting a first cavity and a second cavity; and introducing a connection medium to replace the separation medium and thereby start substance transfer to the second cavity. Substance transfer may be readily controlled without relying on mechanical means. Based on the present invention, two-dimensional electrophoretic analysis can be readily implemented on a chip.
Claims10
A method for controlling the transfer of a fluid substance, and/or a substance comprised in a fluid substance, from a first cavity to a second cavity, comprising the steps of:
- a) introducing into the first cavity a fluid substance whose transfer is to be controlled, and/or a substance whose transfer is to be controlled comprised in the fluid substance, and holding the fluid substance and/or the substance comprised in the fluid substance in the first cavity, wherein the first cavity is connected to the second cavity by an intermediate cavity, and the intermediate cavity is provided with a separation medium which prevents transfer of the fluid substance and/or the substance comprised in the fluid substance into the intermediate cavity;
- b) replacing the separation medium in the intermediate cavity with a connection medium that allows transfer of the fluid substance and/or the substance comprised in the fluid substance into the intermediate cavity;
- and
- c) transferring the fluid substance and/or the substance comprised in the fluid substance from the first cavity to the second cavity via the intermediate cavity.
A device for controlling the transfer of a fluid substance and/or a substance comprised in a fluid substance, comprising:
- a) a first cavity for holding the fluid substance;
- b) a second cavity for holding the fluid substance;
- and
- c) an intermediate cavity for connecting the first cavity and the second cavity, for holding a separation medium which prevents transfer of the fluid substance and/or the substance comprised in the fluid substance from the first cavity;
- wherein the separation medium can be replaced with a connection medium, and the introduction of the connection medium to the intermediate cavity enables transfer of the fluid substance and/or the substance comprised in the fluid substance retained in the first cavity, to the intermediate cavity and the second cavity.
A two-dimensional electrophoretic device comprising;
- a) a first cavity for holding an electrophoretic medium;
- b) a second cavity for holding an electrophoretic medium;
- and
- c) an intermediate cavity for connecting the first cavity and the second cavity, for holding a separation medium which prevents transfer of the substance to be electrophoresed from the first electrophoretic medium to the second electrophoretic medium;
- wherein the separation medium can be replaced with a connection medium, and the introduction of the connection medium into the intermediate cavity enables transfer of the substance to be electrophoresed in the electrophoretic medium retained in the first cavity, to the intermediate cavity and the second cavity.
Dependencies are inferred from the claim text.