Method of producing plants having enhanced transpiration efficiency and plants produced therefrom
OwnerJosette Masle, Graham Farquhar +1inventors
InventorsJosette Masle, Graham Farquhar, Scott Gilmore
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Abstract
The present invention provides methods of selecting plants having modified transpiration efficiency using plant ERECTA gene sequences and nucleic acids linked thereto, and to methods of producing plants having modified transpiration efficiency using isolated plant ERECTA gene sequences, in both traditional plant breeding and genetic engineering approaches. The invention further extends to plants produced by the methods described.
Claims26
A method of selecting a plant having enhanced transpiration efficiency, comprising detecting a genetic marker for transpiration efficiency which marker comprises a nucleotide sequence linked genetically to an ERECTA locus in the genome of the plant and selecting a plant that comprises or expresses the genetic marker.
A method of selecting a plant having enhanced transpiration efficiency, comprising:
- (a) screening mutant or near-isogenic or recombinant inbred lines of plants to segregate alleles at an ERECTA locus;
- (b) identifying a polymorphic marker linked to said ERECTA locus;
- and
- (c) selecting a plant that comprises or expresses the marker.
A method of modulating the transpiration efficiency of a plant comprising introducing an isolated ERECTA gene or an allelic variant thereof or the protein-encoding region thereof to a plant and selecting a plant having a different transpiration efficiency compared to a near-isogenic plant that does not comprise the introduced ERECTA gene or allelic variant or protein-encoding region.
An isolated ERECTA gene from wheat capable of determining or modulating the transpiration efficiency of a plant wherein said isolated ERECTA gene comprises a nucleotide sequence selected from the group consisting of:
- (i) a sequence selected from the group consisting of:
- SEQ ID NO:
- 11, SEQ ID NO:
- 12, SEQ ID NO:
- 13, SEQ ID NO:
- 14, SEQ ID NO:
- 15, SEQ ID NO:
- 16, SEQ ID NO:
- 17, SEQ
- ID NO:
- 18, and SEQ ID NO:
- 19;
- (ii) a sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 20;
- and
- (iii) a sequence that is complementary to (i) or (ii).
An isolated ERECTA gene from maize capable of determining or modulating the transpiration efficiency of a plant wherein said isolated ERECTA gene comprises a nucleotide sequence selected from the group consisting of:
- (i) a sequence selected from the group consisting of:
- SEQ ID NO:
- 21, SEQ ID NO:
- 22, SEQ ID NO:
- 23, SEQ ID NO:
- 24, SEQ ID NO:
- 25, SEQ ID NO:
- 26, SEQ ID NO:
- 27, SEQ ID NO:
- 28, SEQ ID NO:
- 29, SEQ ID NO:
- 30. SEQ ID NO:
- 31, SEQ ID NO:
- 32 SEQ ID NO:
- 33, SEQ ID NO:
- 34, SEQ ID NO:
- 35, SEQ ID NO:
- 36, SEQ ID NO:
- 37, SEQ ID NO:
- 38;
- SEQ ID NO:
- 39, SEQ ID NO:
- 40, SEQ ID NO:
- 41, SEQ
- ID NO:
- 42, SEQ ID NO:
- 43 and SEQ ID NO:
- 44;
- (ii) a sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 45;
- and
- (iii) a sequence that is complementary to (i) or (ii).
26-36. (canceled)
Dependencies are inferred from the claim text.