Canola oil having increased oleic acid and decreased linolenic acid content
AssigneeCargill, Incorporated, a Delaware corporation
InventorsLorin DeBonte, William Hitz
Life of this patent
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Abstract
An endogenous oil extracted from Brassica seeds is disclosed that contains, after crushing and extraction, greater than 86% oleic acid and less than 2.5% α-linolenic acid. The oil also contains less than 7% linoleic acid. The Brassica seeds are produced by plants that contain seed-specific inhibition of microsomal oleate desaturase and microsomal linoleate desaturase gene expression. Such inhibition can be created by cosuppression or antisense technology. Such an oil has a very high oxidative stability in the absence of added antioxidants.
Claims24
1-26. (canceled)
A Brassica plant containing at least one recombinant nucleic acid construct, said at least one construct comprising a seed-specific regulatory sequence operably linked to a wild-type microsomal delta-15 fatty acid desaturase coding sequence, said plant further comprising a mutation in at least one endogenous microsomal delta-12 fatty acid desaturase coding sequence, wherein said plant produces seeds yielding an oil having an oleic acid content of about 80% or greater, an α-linolenic acid content of about 2.5% or less, and an erucic acid content of less than about 2%, said oleic acid content, linolenic acid content, and erucic acid content determined after hydrolysis of said oil.
Seeds of a Brassica plant containing at least one recombinant nucleic acid construct, said at least one construct comprising a seed-specific regulatory sequence operably linked to a wild-type microsomal delta-15 fatty acid desaturase coding sequence, said plant further comprising a mutation in at least one endogenous microsomal delta-12 fatty acid desaturase coding sequence, said seeds yielding an oil having an oleic acid content of about 80% or greater, an α-linolenic acid content of about 2.5% or less, and an erucic acid content of less than about 2%, said oleic acid content, linolenic acid content, and erucic acid content determined after hydrolysis of said oil.
A method of producing an oil from Brassica seeds, comprising the steps of:
- a) crushing seeds produced from at least one Brassica plant, said plant having a seed-specific reduction in microsomal delta-15 fatty acid desaturase gene expression and a mutation in at least one endogenous microsomal delta-12 fatty acid desaturase coding sequence;
- and
- b) extracting said oil from said seeds, said oil having an oleic acid content of about 80% or greater, an α-linolenic acid content of about 2.5% or less, and an erucic acid content of less than about 2%, said oleic acid content, linolenic acid content, and erucic acid content determined after hydrolysis of said oil.
Dependencies are inferred from the claim text.