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AssigneePioneer Hi-Bred International, Inc.
InventorsScott Sebastian, Hong Lu, Feng Han, Donald Kyle, Bradley Hedges, Michael Roach, Paul Stephens, Jean Liu
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The invention relates to methods and compositions for identifying soybean plants that are tolerant, have improved tolerance or are susceptible to Sclerotinia sp. infection (the causative agent of white mold). The methods use molecular genetic markers to identify, select and/or construct disease-tolerant plants or identify and counterselect disease-susceptible plants. Soybean plants that display tolerance or improved tolerance to Sclerotinia sp. infection that are generated by the methods of the invention are also a feature of the invention.
A method of identifying a first soybean plant or germplasm that displays tolerance, improved tolerance, reduced susceptibility or susceptibility to Sclerotinia sp. infection, the method comprising detecting in the first soybean plant or germplasm at least one allele of one or more marker locus that is associated with the tolerance, improved tolerance or susceptibility, wherein the one or more marker locus is selected from the group consisting of:
A method of identifying a first soybean plant or germplasm that displays tolerance, improved tolerance, reduced susceptibility or susceptibility to Sclerotinia sp. infection, the method comprising detecting in the first soybean plant or germplasm at least one allele of one or more marker locus that is associated with the tolerance, improved tolerance, reduced susceptibility or susceptibility, wherein the one or more marker locus is selected from the group consisting of:
A method of producing a soybean plant having tolerance or improved tolerance to Sclerotinia sp. infection, the method comprising introducing an exogenous nucleic acid into a target soybean plant or progeny thereof, wherein the exogenous nucleic acid is derived from a nucleotide sequence that is linked to at least one favorable allele of a marker locus that is associated with tolerance or improved tolerance to Sclerotinia sp. infection, wherein the marker locus is selected from the group consisting of:
A method of producing a soybean plant having tolerance or improved tolerance to Sclerotinia sp. infection, the method comprising introducing two or more exogenous nucleic acids into a target soybean plant or progeny thereof, wherein at least one exogenous nucleic acid is derived from a nucleotide sequence that is linked to at least one favorable allele of a marker locus that is associated with tolerance or improved tolerance to Sclerotinia sp. infection, wherein the marker locus is selected from the group consisting of:
A system for identifying a soybean plant predicted to have tolerance, improved tolerance, reduced susceptibility or susceptibility to Sclerotinia sp. infection, the system comprising:
A composition comprising an amplification primer pair capable of initiating DNA polymerization by a DNA polymerase on a soybean nucleic acid template to generate a soybean marker amplicon, wherein said soybean marker amplicon corresponds to soybean marker SAG1032, S60817-TB, S60239-TB or S60222-TB.
Dependencies are inferred from the claim text.