Thermotolerant phytase for animal feed
AssigneeSyngenta Participations AG
InventorsMichael Lanahan, Scott Betts
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Abstract
The invention provides a synthetic phytase polynucleotide which is optimized for expression in plants and which encodes at thermotolerant phytase, as well as isolated thermotolerant phytase enzyme. Also provided are feed or food products comprising a thermotolerant phytase, and transgenic plants which express the thermotolerant phytase. Further provided are methods for making and using thermotolerant phytases, e.g., a method of using a thermotolerant phytase in feed and food processing.
Claims41
1-87. (canceled)
A method to prepare a transformed plant which expresses a thermotolerant phytase comprising:
- a) introducing into a plant cell an expression cassette comprising a promoter operably linked to a nucleic acid molecule encoding a thermotolerant phytase which retains at least 40% activity after 30 minutes at 60° C. and has a specific activity of greater than 200 U/mg at pH 4.5 and 37° C. so as to yield a transformed plant cell;
- and
- b) obtaining a transgenic plant from the transformed plant cell, which plant expresses ther thermotolerant phytase in the cells of the plant,
- and wherein the thermotolerant phytase comprises the polypeptide sequence depicted in amino acids 20-431 of SEQ ID NO.4 or a conservative variant polypeptide thereof.
A transformed plant comprising an expression cassette comprising a promoter operably linked to a nucleic acid molecule encoding a thermotolerant phytase which retains at least 40% activity after 30 minutes at 60° C. and has a specific activity of greater than 200 U/mg at pH 4.5 and 37° C.,
- and wherein the thermotolerant phytase comprises the polypeptide sequence depicted in amino acids 20-431 of SEQ ID NO.4 or a conservative variant polypeptide thereof.
122-124. (canceled)
127-129. (canceled)
Dependencies are inferred from the claim text.