Root-preferred, wound-and insect-inducible 2-oxoglutarate-dependent oxygenase promoter from maize and its use

A method for expressing a nucleotide sequence in a plant, said method comprising introducing into a plant a DNA construct, said DNA construct comprising a promoter and operably linked to said promoter a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:

• a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
• 1;
• b) a nucleotide sequence comprising the plant promoter sequences of the plasmids designated as Patent Deposit No. PTA-6278;
• c) a nucleotide sequence comprising at least 20 contiguous nucleotides of the sequence set forth in SEQ ID NO:
• 1, wherein said nucleotide sequence initiates transcription in said plant;
• and
• d) a nucleotide sequence comprising a sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:
• 1, wherein said nucleotide sequence initiates transcription in a plant cell.

A method for expressing a nucleotide sequence in a plant cell, said method comprising introducing into a plant cell a DNA construct comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:

• a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
• 1;
• b) a nucleotide sequence comprising the plant promoter sequences of the plasmids designated as Patent Deposit No. PTA-6278;
• c) a nucleotide sequence comprising at least 20 contiguous nucleotides of the sequence set forth in SEQ ID NO:
• 1, wherein said nucleotide sequence initiates transcription in said plant cell;
• and
• d) a nucleotide sequence comprising a sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:
• 1, wherein said nucleotide sequence initiates transcription in a plant cell.

A method for selectively expressing a nucleotide sequence in a plant root, said method comprising introducing into a plant cell a DNA construct, and regenerating a transformed plant from said plant cell, said DNA construct comprising a promoter and a heterologous nucleotide sequence operably linked to said promoter, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:

• a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
• 1;
• b) a nucleotide sequence comprising the plant promoter sequences of the plasmids deposited as Patent Deposit No. PTA-6278;
• c) a nucleotide sequence comprising at least 20 contiguous nucleotides of the sequence set forth in SEQ ID NO:
• 1, wherein said sequence initiates transcription in a plant root cell;
• and
• d) a nucleotide sequence comprising a sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:
• 1, wherein said sequence initiates transcription in a plant root cell.