Cytokinin-sensing histidine kinases and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsRobert Meister, Sobhana Sivasankar
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Abstract
Isolated polynucleotides that encode cytokinin-sensing histidine kinase polypeptides, and the encoded polypeptides, are described. Expression cassettes comprising the polynucleotides of the invention and plants and plant cells that are transformed with the polynucleotides are described. Methods of using the cytokinin-sensing histidine kinase polypeptides and polynucleotides to modulate histidine kinase activity and/or histidine kinase levels in plants and plant cells are further described.
Claims20
An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising SEQ ID NO:
- 1, 3, 4, 6, 7, 9, 13, 15, 16, 18, 26, 28, 30, or 32;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2, 5, 8, 14, 17, 27, or 31;
- (c) a nucleotide sequence 90% identical to SEQ ID NO:
- 1, 3, 7, 9, 13, 15, 16, 18, 26, or 28;
- (d) a nucleotide sequence encoding a polypeptide comprising at least 50 consecutive amino acids of SEQ ID NO:
- 2, 8, 14, 17, or 27, wherein said polypeptide retains histidine kinase activity;
- and
- (e) a nucleotide sequence that hybridizes under stringent conditions to the complement of SEQ ID NO:
- 1, 3, 7, 9, 13, 15, 16, 18, 26, or 28, wherein said stringent conditions comprise hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and at least one wash in 0.1×SSC at 60° C. to 65° C.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence comprising SEQ ID NO:
- 2, 5, 8, 14, 17, 23, 27, or 31;
- (b) an amino acid sequence comprising at least 70% sequence identity to SEQ ID NO:
- 2, 8, 14, 17, 23, or 27, wherein said polypeptide retains histidine kinase activity;
- (c) an amino acid sequence encoded by a nucleotide sequence that hybridizes under stringent conditions to the complement of 1, 3, 7, 9, 13, 15, 16, 18, 26 or 28, wherein said stringent conditions comprise hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and at least one wash in 0.1×SSC at 60° C. to 65° C.;
- and,
- (d) an amino acid sequence comprising at least 50 consecutive amino acids of SEQ ID NO:
- 2, 8, 14, 17, 23, or 27, wherein said polypeptide retains kinase activity.
A transformed plant comprising a polynucleotide operably linked to a promoter that drives expression in a plant, wherein said polynucleotide comprises a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising SEQ ID NO:
- 1, 3, 4, 6, 7, 9, 13, 15, 16, 18, 26, 28, 30, or 32;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2, 5, 8, 14, 17, 23, 27, or 31;
- (c) a nucleotide sequence comprising at least 70% sequence identity to SEQ ID NO:
- 1, 3, 7, 9, 13, 15, 16, 18, 26, or 28, wherein said polynucleotide encodes a polypeptide with histidine kinase activity;
- (d) a nucleotide sequence encoding an amino acid sequence comprising at least 70% sequence identity to SEQ ID NO:
- 2, 8, 14, 17, or 27, wherein said polynucleotide encodes a polypeptide comprising histidine kinase activity;
- (e) a nucleotide sequence that hybridizes under stringent conditions to the complement of SEQ ID NO:
- 1, 3, 7, 9, 13, 15, 16, 18, 26, or 28, wherein said stringent conditions comprise hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and at least one wash in 0.1×SSC at 60° C. to 65° C.;
- and
- a nucleotide sequence that is fully complementary to at least one sequence selected from the group consisting of the nucleotide sequences of (a)-(e).
A method for modulating the level or activity of a polypeptide in a plant comprising transforming said plant with a construct comprising a fragment of SEQ ID NO:
- 1, 3, 4, 6, 7, 9, 13, 15, 16, 18, 26, 28, 30, or 32, or a fragment of the complement of any of the same, wherein expression of said fragment disrupts transcription or translation of the corresponding endogenous polynucleotide or an endogenous polynucleotide 90% identical thereto.
Dependencies are inferred from the claim text.