Modified hemoglobin and methods of making same
AssigneeAlbert Einstein College of Medicine of Yeshiva University
InventorsSeetharama Acharya, Belur Manjula
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Abstract
The present invention provides a hemoglobin molecule (Hb) having six±one PEG chains, wherein two of said PEG chains are bound to Cys-93 (β) of Hb, and the remaining PEG chains are bound to thiol groups introduced on ε-NH2 of Hb. The present invention also provides a process for preparing a modified hemoglobin molecule (Hb), comprising the steps of: (a) reacting Hb with 8-15 fold excess of iminothiolane to form thiolated Hb; and (b) reacting the thiolated Hb with 16-30 fold excess of PEG functionalized with a maleimide moiety, to form the modified Hb.
Claims27
A hemoglobin molecule (Hb) having six±one PEG chains, wherein two of said PEG chains are bound to Cys-93(β) of Hb, and the remaining PEG chains are bound to thiol groups introduced on ε-NH2 of Hb.
A process for preparing a modified hemoglobin molecule (Hb), comprising the steps of:
- (a) reacting Hb with 8-15 fold excess of iminothiolane to form thiolated Hb;
- and
- (b) reacting the thiolated Hb with 16-30 fold excess of PEG functionalized with a maleimide moiety, to form the modified Hb.
Dependencies are inferred from the claim text.