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US 2006/0135546 A1Published application
Methods for the purification of 20(S)- camptothecin
OwnerJagadevappa Basavaraja, Sudhir Chellappa +2inventors
InventorsJagadevappa Basavaraja, Sudhir Chellappa, Vijay Shastry, Rao Subba
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Abstract
The invention features scalable methods for the purification of (20S)-camptothecin.
Claims20
A method of producing enriched camptothecin from crude camptothecin, said method comprising:
- a) combining solid crude camptothecin with a liquid in which camptothecin is insoluble or sparingly soluble to form a mixture containing dissolved impurities;
- b) separating the undissolved material from the liquid;
- and
- c) repeating steps (a) and (b) until said undissolved material is enriched camptothecin.
1 more element
A method of producing material containing 98% or greater camptothecin from enriched camptothecin, said method comprising:
- a) combining said enriched camptothecin with glacial acetic acid;
- b) heating the combination of step (a) to produce a solution having camptothecin dissolved therein;
- c) cooling said solution to form a precipitate from dissolved camptothecin;
- and
- d) separating said precipitate from said solution, wherein said precipitate contains 98% or greater camptothecin.
2 more elements
A method of producing material containing 98% or greater camptothecin from enriched camptothecin, said method comprising:
- a) loading enriched camptothecin onto a column packed with an adsorbent material;
- b) passing a mobile phase of chloroform followed by chloroform/acetone mixtures across said adsorbent material;
- c) collecting the eluent in fractions;
- and
- d) combining camptothecin rich fractions and separating the dissolved solids therein to produce a material containing 98% or greater camptothecin.
2 more elements
A method of purifying crude camptothecin, said method comprising:
- a) combining solid crude camptothecin with a liquid in which camptothecin is insoluble or sparingly soluble to form a mixture containing dissolved impurities;
- b) separating the undissolved material from the liquid;
- c) repeating steps (a) and (b) until said undissolved material is enriched camptothecin;
- and
- d) recrystallizing said enriched camptothecin from a solvent to form a precipitate, wherein said precipitate contains 98% or greater camptothecin.
2 more elements
A method of purifying crude camptothecin, said method comprising:
- a) combining solid crude camptothecin with a liquid in which camptothecin is insoluble or sparingly soluble to form a mixture containing dissolved impurities;
- b) separating the undissolved material from the liquid;
- c) repeating steps (a) and (b) until said undissolved material is enriched camptothecin;
- d) loading enriched camptothecin onto a column packed with an adsorbent material;
- e) passing a mobile phase across said adsorbent material;
- f) collecting the eluent in fractions;
- and
- g) combining camptothecin rich fractions and separating the dissolved solids therein to produce a material containing 98% or greater camptothecin.
5 more elements
Dependencies are inferred from the claim text.