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US 2006/0135457 A1Published application
Methods for constructing antibiotic resistance free vaccines
OwnerYvonne Paterson, Thorsten Verchinventors
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Abstract
The present invention provides DNA vaccines comprising an antibiotic resistance gene-free plasmid, methods of generating same, and methods for treating a disease agent, comprising same.
Claims30
A DNA vaccine for generating an immune response against a protein antigen, said DNA vaccine comprising an antibiotic resistance gene-free plasmid, said antibiotic resistance gene-free plasmid comprising:
- a first nucleic acid sequence encoding a polypeptide that comprises said protein antigen;
- and
- a second nucleic acid sequence encoding a metabolic enzyme,
- wherein said antibiotic resistance gene-free plasmid is grown in an auxotrophic bacterial strain, whereby said metabolic enzyme complements a metabolic deficiency of said auxotrophic bacterial strain, thereby generating an immune response against a protein antigen.
1 more element
A method of preparing a DNA vaccine for generating an immune response against a protein antigen, said method comprising
- a. growing an auxotrophic bacterial strain containing a plasmid, wherein said plasmid comprises:
- first nucleic acid sequence encoding a polypeptide that comprises said protein antigen;
- and
- a second nucleic acid sequence encoding a metabolic enzyme, whereby said metabolic enzyme complements a metabolic deficiency of said auxotrophic bacterial strain;
- and wherein said plasmid does not contain an antibiotic resistance gene;
- and
- b. and isolating the plasmid DNA vaccine from said auxotrophic bacterial strain,
- thereby preparing a DNA vaccine for generating an immune response against a protein antigen.
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Dependencies are inferred from the claim text.