Vaccine
OwnerSara Brett, Paul Hamblin +1inventors
InventorsSara Brett, Paul Hamblin, Louise Ogilvie
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Abstract
The present invention relates to methods and compositions useful in the treatment and prevention of Hepatitis C virus (HCV) infections and the symptoms and diseases associated therewith. In particular the present invention relates to DNA vaccines that encode the HCV Core protein and a polynucleotide sequence that encodes at least one other HCV protein, wherein the vaccine causes expression of the proteins within the same cell and the sequence of the polynucleotide sequence encoding the core protein has been mutated or positioned relative to the polynucleotide sequence encoding the at least one other HCV protein such that the negative effect of expression of the Core protein upon the expression of the said at least one other HCV protein is reduced.
Claims20
A polynucleotide vaccine comprising a polynucleotide sequence that encodes an HCV Core protein and a polynucleotide sequence that encodes at least one other HCV protein, wherein the polynucleotide vaccine causes expression of the Core protein and other HCV proteins within the same cell, wherein the Core protein and the at least one other HCV protein are encoded in more than one expression cassette, wherein a first expression cassette encoding the Core protein is in a cis location downstream of a second expression cassette that encodes at least one of the other HCV proteins.
A polynucleotide vaccine comprising a polynucleotide sequence that encodes an HCV Core protein and a polynucleotide sequence that encodes at least one other HCV protein, wherein the vaccine causes expression of the Core protein and other HCV proteins within the same cell and the sequence of the polynucleotide sequence encoding the Core protein has been mutated, wherein the mutation reduces expression of the Core protein upon the expression of said at least one other HCV protein, and the Core protein and other HCV proteins are encoded by the polynucleotide vaccine in more than one expression cassette.
The polynucleotide vaccine as claimed in 3, wherein the truncated Core protein has a deletion of at least the C-terminal 10 amino acids.
(canceled)
The polynucleotide vaccine comprising a polynucleotide sequence that encodes an HCV Core protein and a polynucleotide sequence that encodes at least one other HCV protein, wherein the polynucleotide vaccine causes expression of the Core protein and other HCV proteins within the same cell and the sequence of the polynucleotide sequence encoding the Core protein has been mutated or positioned relative to the polynucleotide sequence encoding the at least one other HCV protein, wherein the mutation reduces expression of the Core protein upon the expression of said at least one other HCV protein, wherein the Core protein encoded by the polynucleotide vaccine consists of one of the following group of sequences encoding:
- amino acids 1-151 of the Core protein, amino acids 1-165 of the Core protein, and amino acids 1-171 of the Core protein.
(canceled)
Dependencies are inferred from the claim text.