Novel method of selecting immunosuppressant having little thrombocytopenic effect

A method for selecting an immunosuppressive agent with a less thrombocytopenia effect, said method comprising the following items (1) to (3):

• (1) measuring an immunosuppressive activity of an analyte;
• (2) measuring a GATA-1 transcription inhibitory activity of the analyte;
• and
• (3) comparing the immunosuppressive activity determined in (1) with the GATA-1 transcription inhibitory activity determined in (2) to select an immunosuppressive agent with a less thrombocytopenia effect.

A method for selecting an immunosuppressive agent with a less thrombocytopenia effect, said method comprising the following items (1) to (3):

• (1) measuring an IL-2 transcription inhibitory activity in a test cell in the coexistence of the test cell and an analyte;
• (2) measuring an GATA-1 transcription inhibitory activity in a test cell in the coexistence of the test cell and an analyte;
• and
• (3) comparing the IL-2 transcription inhibitory activity determined in (1) with the GATA-1 transcription inhibitory activity determined in (2) to select an immunosuppressive agent with a less thrombocytopenia effect.

A method for selecting an immunosuppressive agent with a less thrombocytopenia effect, said method comprising the following items (1) to (3):

• (1) measuring an IL-2 transcription inhibitory activity in a test cell into which an IL-2 reporter gene has been introduced in the coexistence of the test cell and an analyte;
• (2) measuring a GATA-1 transcription inhibitory activity in a test cell into which a GATA-1 reporter gene has been introduced in the coexistence of the test cell and an analyte;
• and
• (3) comparing the IL-2 transcription inhibitory activity determined in (1) with the GATA-1 transcription inhibitory activity determined in (2) to select an immunosuppressive agent with a less thrombocytopenia effect.

A method for selecting an immunosuppressive agent with a less thrombocytopenia effect, said method comprising the following items (1) to (3):

• (1) measuring the amount of expression of IL-2 protein;
• (2) measuring the amount of expression of GATA-1 protein;
• and
• (3) comparing both the amounts of expression to select an immunosuppressive agent with a less thrombocytopenia effect.

A kit for measurement in selecting an immunosuppressive agent with a less thrombocytopenia effect, comprising the following items (1) to (4):

• (1) a DNA construct containing an IL-2 reporter gene;
• (2) a DNA construct containing a GATA-1 reporter gene;
• (3) a test cell of T-cell line;
• and
• (4) a test cell of megakaryocytic cell line.