EGVII endoglucanase and nucleic acids encoding the same
AssigneeDaniaco A/S, Genencor Division
InventorsNigel Dunn-Coleman, Frits Goedegebuur, Michael Ward, Jian Yao
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Abstract
The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.
Claims5
1-17. (canceled)
A substantially purified EGVII polypeptide with the biological activity of an endoglucanase, comprising a sequence selected from the group consisting of:
- (a) an amino acid sequence having at least 98% sequence identity to the amino acid sequence SEQ ID NOs:3 and 2 as shown in FIG. 2;
- (b) an amino acid sequence having at least 98% sequence identity to the amino acid sequence SEQ ID NO:2;
- (c) an amino acid sequence having at least 95% sequence identity to the amino acid sequence SEQ ID NOs:3 and 2 as shown in FIG. 2;
- (d) an amino acid sequence SEQ ID NOs:3 and 2 as shown in FIG. 2-in FIG. 2;
- (e) an amino acid sequence having at least 95% sequence identity to the amino acid sequence presented as SEQ ID NO:2;
- (f) an amino acid sequence presented as SEQ ID NO:2;
- (g) a substantially purified biologically active fragment of the amino acid sequence presented as SEQ ID NO:2.
19-20. (canceled)
A purified enzyme having endoglucanase activity prepared by:
- (a) stably transforming a host cell with an expression vector comprising a polynucleotide selected from the group consisting of:
- (i) a nucleic acid sequence which encodes or is a full-length complement to a sequence which encodes an EGVII polypeptide having at least 98% sequence identity to the amino acid sequence SEQ ID NOs:3 and 2 as shown in FIG. 2;
- (ii) a nucleic acid sequence which encodes or is complementary to a sequence which encodes an EGVII polypeptide having at least 95% sequence identity to the amino acid sequence presented in SEQ ID NOs:3 and 2 as shown in FIG. 2;
- (iii) a nucleic acid sequence which encodes or is complementary to a sequence which encodes an EGVII polypeptide having the amino acid sequence presented in SEQ ID NOs:3 and 2 as shown in FIG. 2;
- (iv) a nucleic acid sequence which encodes or is complementary to a sequence which encodes an EGVII polypeptide having at least 95% sequence identity to the amino acid sequence presented as SEQ ID NO:2;
- (v) a nucleic acid sequence which encodes or is complementary to a sequence which encodes an EGVII polypeptide having the amino acid sequence SEQ ID NO:2;
- and
- (vi) a nucleic acid sequence SEQ ID NO:4, or the full-length complement thereof;
- wherein % identity is calculated using the CLUSTAL-W program in MacVector version 6.5, operated with default parameters, including an open gap Penalty of 10.0 an extended gap penalty of 0.1, and a BLOSUM 30 similarity matrix;
- (b) cultivating said transformed host cell under condition suitable for said host cell to produce said endoglucanase;
- and
- (c) recovering said endoglucanase.
22-36. (canceled)
Dependencies are inferred from the claim text.