Method of separating unattached Raman-active tag from bioassay or other reaction mixture
AssigneeGeneral Electric Company
InventorFrank Mondello
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Abstract
A method of separating a Raman-active tag unattached to a target from a Raman-active complex is disclosed. The Raman-active complex includes a Raman-active tag attached to a target. The method includes providing a mixture including at least one Raman-active tag unattached to a target and at least one Raman-active complex; and passing the mixture through a porous membrane.
Claims44
A method of separating a Raman-active tag unattached to a target from a Raman-active complex, wherein the Raman-active complex comprises a Raman-active tag attached to a target,
- the method comprising:
- i) providing a mixture comprising at least one Raman-active tag unattached to a target and at least one Raman-active complex,
- wherein the Raman-active complex has a first size;
- ii) passing the mixture through a porous membrane,
- wherein the membrane comprises pores having an effective size smaller than the first size.
A method of separating a Raman-active tag unattached to a target from a Raman-active complex, wherein the Raman-active complex comprises a Raman-active tag attached to a target,
- the method comprising:
- i) providing a mixture comprising at least one Raman-active tag unattached to a target and at least one Raman-active complex,
- wherein the Raman-active complex has a first size;
- ii) passing the mixture through a porous membrane,
- wherein the membrane comprises pores having an effective size smaller than the first size.
- iii) applying a sufficient force to the membrane to at least separate one Raman-active complex from the membrane while the Raman-active tag unattached to a target remains on the membrane.
A method of separating a Raman-active tag unattached to a target from a Raman-active complex, wherein the Raman-active complex comprises a Raman-active tag attached to a target,
- the method comprising:
- i) providing a mixture comprising at least one Raman-active tag unattached to a target and at least one Raman-active complex,
- wherein the Raman-active complex has a first size;
- ii) passing the mixture through a porous membrane comprising pores,
- wherein the porous membrane is configured to collect at least one Raman-active complex.
Dependencies are inferred from the claim text.