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US 2006/0134802 A1Published application
Solid-phase immunochromatographic methods
AssigneeVedalab
InventorsRaphael Donati, Patrick Bigot
Life of this patent
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Abstract
The invention concerns solid-phase immunochromatographic methods for detecting an analyte in a liquid sample. In the inventive methods, a binding reagent conjugated with a particulate marker is extemporaneously added in liquid form.
Claims14
1-13. (canceled)
A method for detecting an analyte in a liquid sample, comprising the following steps:
- a) a porous solid support provided with a collection zone and a detection zone is provided, a capture reagent being immobilized in the detection zone;
- b) the following are deposited, separately, successively and extemporaneously, in the collection zone of the porous solid support:
- i) a binding reagent conjugated to a particulate label, the reagent being in liquid form,
- ii) the liquid sample,
- c) an amount of time sufficient for the migration, by capillary diffusion, of the binding reagent conjugated to a particulate label and of the liquid sample from the collection zone to the detection zone of the porous solid support is allowed to elapse,
- d) the extent to which the binding reagent conjugated to a particulate label attaches in the detection zone is observed.
3 more elements
A method for detecting an analyte in a liquid sample, comprising the following steps:
- a) a porous solid support provided with a collection zone and a detection zone is provided, a capture reagent being immobilized in the detection zone;
- b) the following are deposited, separately, successively and extemporaneously, in the collection zone of the porous solid support:
- i) a binding reagent conjugated to a particulate label, the reagent being in liquid form,
- ii) the liquid sample,
- iii) a diluent in liquid form,
- c) an amount of time sufficient for the migration, by capillary diffusion, of the binding reagent conjugated to a particulate label, of the liquid sample and of the diluent from the collection zone to the detection zone of the porous solid support is allowed to elapse,
- d) the extent to which the binding reagent conjugated to a particulate label attaches in the detection zone is observed.
4 more elements
A method for detecting an analyte in a liquid sample, comprising the following steps:
- a) a porous solid support provided with a collection zone and a detection zone is provided, a capture reagent being immobilized in the detection zone;
- b) the following are deposited, separately, successively and extemporaneously, in the collection zone of the porous solid support:
- i) the liquid sample,
- ii) a binding reagent conjugated to a particulate label, the reagent being in liquid form,
- iii) a diluent in liquid form,
- c) an amount of time sufficient for the migration, by capillary diffusion, of the binding reagent conjugated to a particulate label, of the liquid sample and of the diluent from the collection zone to the detection zone of the porous solid support is allowed to elapse,
- d) the extent to which the binding reagent conjugated to a particulate label attaches in the detection zone is observed.
4 more elements
Dependencies are inferred from the claim text.