Method and apparatus for automated pre-treatment and processing of biological samples

A method for automated de-paraffinization, antigen retrieval and staining of a biological sample on a slide, comprising the steps of:

• a.) providing an apparatus comprising a heatable processing tank for performing de-paraffinization and antigen retrieval upon the sample, a slide holder for holding the slide and a moveable probe for administering reagents to the sample during staining;
• b.) mounting the slide onto the slide holder;
• c.) causing the sample to contact a first solution within the processing tank, the first solution causing de-paraffinization of the sample;
• d.) draining the first solution from the processing tank;
• e.) causing the sample to contact a second solution heated within the processing tank;
• f.) removing the slide from the processing tank after a duration of time appropriate for antigen retrieval;
• and
• g.) administering the reagents to the sample with the probe,
• wherein the second solution comprises at least one chelating agent, at least one detergent and at least 50% by volume of at least one liquid that facilitates antigen retrieval.