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AssigneeGenein Co., Ltd.
InventorsCheol-Min Kim, Hee-Kyung Park, Mong Cho, Hyun-Jung Jang, Jeong Heo
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Provided are a microarray manufactured using a mixture of target probes for drug-resistant HBV detection, quality control probes for controlling quality in probe hybridization and fabrication of microarrays, and negative control probes for determining the presence and ratio of more than one type, i.e., a wild type and a mutant in a codon, measuring a background of non-specific cross-hybridization, and discriminating homozygotes and heterozygotes, and a method of detecting a drug-resistant HBV, controlling the quality of a microarray, determining the presence and ratio of more than one type, and determining positive and false positive probes at the same time using the microarray. The microarray, which includes the target probes for drug-resistant HBV detection, the QC probes, and the negative control probes, can detect a drug-resistant HBV, control quality in fabrication of microarrays and hybridization, determine the presence and ratio of more than one type, i.e., a wild type and a mutant, determine positive and false positive probes by measuring a background of non-specific cross-hybridization, and discriminate homozygotes and heterozygotes. When a plurality of sets of probes, each set containing target probes, QC probes, and negative control probes, are immobilized on a support of the microarray, detection of resistance in HBV to multiple drugs, quality control, and determination as to the presence and ratio of a wild type and a mutant and whether each probe is positive or false positive can be rapidly and accurately performed.
A microarray with target probes for detecting drug-resistant HBV on a support, wherein the target probes comprise oligonucleotides including the nucleotide sequences of point mutations at codons 528, 529, and 514 in domain B and at codons 552, 548, and 555 in domain C of a HBV DNA polymerase gene that induce resistance to lamivudine and/or oligonucleotides including the nucleotide sequences of point mutations at codons 528 and 529 in domain B and at codon 555 in domain C of the HBV DNA polymerase gene that induce resistance to famciclovir.
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A primer or probe for detecting HBV drug resistance, the primer or probe comprising one of nucleotide sequences having SEQ ID NOs. 1 through 47.
A negative control probe for detecting the presence and ratio of more than one type, detecting positive and false positive probes by measuring a background of non-specific cross-hybridization, discriminating homozygotes and heterozygotes, and/or genotyping, the negative control probe being prepared by substituting, inserting, or deleting at least one nucleotide sequence among the nucleotide sequences of the target probes for detecting drug-resistant HBV that have one of nucleotide sequences of SEQ ID NOs. 7 through 47.
Dependencies are inferred from the claim text.