Methods and products for biasing cellular development
AssigneeUniversity of Central Florida Research Foundation, Inc.
InventorsKiminobu Sugaya, Amelia Marutle, Angel Alvarez
Life of this patent
Estimating the term…
Abstract
Methods are described that bias cells, such as potent and multipotent stem cells, by transfection with a nucleic acid sequence, to differentiate to a desired end-stage cell or a cell having characteristics of a desired end-stage cell. In particular embodiments, human neural stem cells are transfected with vectors comprising genes in the homeobox family of transcription factor developmental control genes, and this results in a greater percentage of resultant transformed cells, or their progeny, differentiating into a desired end-stage cell or a cell having characteristics of a desired end-stage cell.
Claims15
A method of biasing differentiation of a neural stem cell comprising introducing a nucleic acid sequence into the neural stem cell, the nucleic acid sequence comprising a promoter operatively linked to an expressible sequence that specifically hybridizes under highly stringent conditions to a complement of an identified sequence selected from the group consisting of SEQ ID NOs:1-8, 9 and 13, the nucleic acid sequence comprising a transcription termination site, wherein expression of the expressible sequence is effective to bias the neural stem cell to a desired end-stage cell type, or to an presumptive end-stage cell having characteristics of the desired end-stage cell type.
A method of biasing differentiation of a neural stem cell comprising:
- a. providing the neural stem cell;
- b. preparing a nucleic acid sequence comprising a promoter operatively linked to an expressible sequence that specifically hybridizes under highly stringent conditions to a complement of an identified sequence selected from the group consisting of SEQ ID NOs:1-8, 9 and 13, the nucleic acid sequence comprising a transcription termination site;
- and
- c. step for transfecting said neural stem cell with said nucleic acid sequence;
- wherein expression of the expressible sequence results in biasing the neural stem cell to a desired end-stage cell type, or to an presumptive end-stage cell having characteristics of the desired end-stage cell type.
A method of biasing differentiation of one or more cells in a population of cells comprising multipotent stem cells, comprising:
- a. providing the population of cells in a vessel;
- b. adding to the vessel one or more copies of a nucleic acid sequence into the neural stem cell, each nucleic acid sequence comprising a promoter operatively linked to a expressible sequence, the expressible sequence specifically hybridizing under highly stringent conditions to a complement of a sequence selected from the group consisting of SEQ ID NOs:1-9, 12 and 13, and a transcription termination site,
- wherein expression of the expressible sequence in one or more cells transfected with a copy of the nucleic acid sequence is effective to bias the one or more cells to a desired end-stage cell type, or to an presumptive end-stage cell having characteristics of the desired end-stage cell type.
A method of modifying a potent cell to bias differentiation of said potent cell toward a desired end-stage differentiated cell comprising the step of:
- introducing a nucleic acid sequence into said potent cell, the nucleic acid sequence comprising a promoter operatively linked to a developmental control gene selected from the group consisting of SEQ ID NOs:1-9, 12, and 13 and variant nucleic acid sequences hybridizable to SEQ ID NOs:1-9, 12, and 13;
- wherein expression of the developmental biasing gene increases probability of said potent cell to differentiate into a desired end-stage differentiated cell.
A method of modifying a potent cell to bias differentiation of said potent cell toward a desired end-stage differentiated cell comprising the step of:
- introducing a nucleic acid sequence into said potent cell, the nucleic acid sequence comprising a promoter operatively linked to a developmental control gene selected from the group consisting of SEQ ID NOs:1-9, 12, and 13 and variant nucleic acid sequences hybridizable to SEQ ID NOs:9, 12, and 13;
- wherein expression of the developmental biasing gene increases probability of said potent cell to differentiate into a desired end-stage differentiated cell.
Dependencies are inferred from the claim text.