Method of treating a malignancy in a subject via direct picornaviral-mediated oncolysis
Assigneevirotarg pty limited
InventorDarren Shafren
Life of this patent
Estimating the term…
Abstract
There are provided methods for treatment of abnormal cells such as cancer cells in a mammal. The methods involve treating the mammal with virus selected from echoviruses and modified forms and combination thereof, which recognise α2β1 for infectivity of the cells. There are also provided methods for screening viruses for use in a method of the invention as well as pharmaceutical compositions for use in the methods.
Claims63
A method for of treating abnormal cells in a mammal comprising administering to the mammal an effective amount of virus selected from echo viruses, and modified forms and combinations thereof, which recognize α2β1 for infectivity of the cells such that at least one of the cells are killed by the virus.
A method of screening a sample of abnormal cells from a mammal for susceptibility to virus induced cell death to evaluate administering virus to the mammal for treatment of the abnormal cells, the method comprising:
- (a) contacting the cells with a virus for a period of time sufficient to allow infection of the cells by the virus;
- and
- (b) determining whether the virus has infected the cells and caused death of at least one of the abnormal cells;
- wherein the virus is selected from echo viruses, and modified forms and combinations thereof, which recognize α2β1 for infectivity of the abnormal cells.
A method of screening a virus for ability to infect and cause death of abnormal cells from a mammal to evaluate administering the virus to the mammal for treatment of the abnormal cells, the method comprising:
- (a) contacting abnormal cells from the mammal with a virus for a period of time sufficient to allow infection of the cells by the virus;
- and
- (b) determining whether the virus has infected and caused death of at least one of the abnormal cells;
- wherein the virus is selected from echoviruses and modified forms thereof, which recognize α2β1 for infectivity of the abnormal cells.
A method for inducing an immune response in a mammal against abnormal cells expressing α2β1, the method comprising infecting abnormal cells in the mammal with a virus selected from the group consisting of echoviruses, modified forms thereof, and combinations thereof, thereby causing lysis of at least one of the cells and inducing an immune response in the mammal against the abnormal cells.
A pharmaceutical composition for treating abnormal cells in a mammal, comprising an inoculant for generating virus to treat the cells such that at least one of the cells is killed by the virus, and a pharmaceutically acceptable carrier, wherein the virus recognizes α2β1 for infectivity of the cells and is selected from echoviruses, modified forms thereof, and combinations thereof.
An applicator for applying an inoculant to a mammal for generating a virus to treat abnormal cells in the mammal, wherein the applicator comprises a region impregnated with the inoculant, and the virus recognizes α2β1 for infectivity of the cells and is selected from echoviruses, modified forms thereof, and combinations thereof.
(canceled)
Dependencies are inferred from the claim text.