Detection of protease enzymes
AssigneeMarligen Biosciences, Inc.
InventorsPeter Clausen, James Lazar
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Abstract
Methods and compositions for the detection of biomolecules, for example, proteases, are provided. The novel compositions, methods, and kits of the present invention have broad applicability in the detection of proteases, and providing enhanced specificity in the detection of proteases. The compositions and methods may be used to measure the activities of multiple proteases simultaneously or in a multiplexed format, particularly in planar and liquid array formats.
Claims30
A detectable composition comprising a detectable complex immobilized upon a capture surface, wherein said detectable complex comprises a protease bound to a labeled inhibitor.
A multiplex detection system, comprising a substrate subdivided into a plurality of distinct loci, wherein each locus comprises a detectable complex immobilized on the surface of said locus, and wherein said detectable complex comprises a protease bound to a labeled inhibitor.
The system according to 7, wherein said labeled inhibitor is labeled with a moiety selected from the group consisting of colorimetric labels, fluorescent labels, chemiluminescent labels, bioluminescent labels, biotin, digoxigenin, detectable carbohydrates, oligonucleotides, nucleic acids, peptides, polypeptides, protein, and glycoproteins.
A method of detecting a protease in a sample, comprising detecting the presence of a labeled complex on a capture surface, wherein said labeled complex is derived from said sample and comprises a protease bound to a labeled inhibitor.
A method of detecting a plurality of proteases, comprising detecting a plurality of labeled complexes compositions on a plurality of capture surface, wherein each labeled complex comprises a protease bound to a labeled inhibitor.
Dependencies are inferred from the claim text.