Gene cluster involved in safracin biosynthesis and its uses for genetic engineering
AssigneePharmaMar, S.A.
InventorsAna Velasco Iglesias, Fernando De La Calle, Tomas Aparicio Perez, Carmen Schleissner Sanchez, Paloma Acebo Pais, Pilar Rodriguez Ramos, Fernando Reyes Benitez, Ruben Henriquez Pelaez
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Abstract
A gene cluster has open reading frames which encode polypeptides sufficient to direct the synthesis of a safracin molecule.
Claims42
A gene cluster having open reading frames which encode polypeptides sufficient to direct the synthesis of a safracin molecule.
A nucleic acid sequence comprising:
- a) a nucleic acid sequence encoding at least one non-ribosomal peptide synthetase which catalyse at least one step of the biosynthesis of safracins;
- b) a nucleic acid sequence which is complementary to the sequence in a);
- or
- c) variants or portions of the sequences of a) or b).
A hybridization probe comprising a nucleic acid sequence according to any one of the preceding claims.
A method of producing a safracin compound or safracin analogue comprising fermenting an organism in which expression of genes encoding polypeptides sufficient to direct the synthesis of a safracin or safracin analogue has been modulated by manipulation or replacement of one or more genes or sequence responsible for regulating such expression.
Use of P2, P14, analogs and derivatives thereof in combinatorial biosynthesis of one or more of non-ribosomal peptide synthetases, diketopiperazine rings and safracins.
Dependencies are inferred from the claim text.