Novel endophytic camptothecin and camptothecinoid producing fungi and process of producing the same
AssigneeCouncil of Scientific and Industrial Research
InventorsSatish Puri, Vijeshwar Verma, Touseef Amna, Geeta Handa, Vinay Gupta, Neelam Verma, Ravi Khajuria, Ajit Saxena, Ghulam Qazi, Michael Spiteller
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Abstract
The present invention relates to a novel source of microorganism for production of Camptothecin and related camptothecinoids. The invention also discloses its isolation, screening for Camptothecin production, growth, fermentation requirements and chemical analysis of Camptothecin (camptothecinoids).
Claims17
A novel endophytic fungal strain having accession no. MTCC 5124, deposited at International Depository at Institute of Microbial technology (IMTECH), Chandigarh, New Delhi, for production of Camptothecin and Camptothecinioids (CPT), said strain has following characteristics:
- (a) fungal strain forms white colony mycelium and turns black during sporulation,
- (b) forms aerial hyphae, which are round to globose and terminal.
A process of isolating Camptothecin and Camptothecinioids (CPT) from novel endophytic fungal strain having accession no. MTCC 5124, said process comprising the steps of:
- (a) incubating the fungal strain on to a carbohydrate medium for about 6-7 days at temperature of 28±2° C. at 220-220 rpm,
- (b) filtering the fungal mycelia through whatman paper under vacuum to obtain the fungal pellet,
- (c) washing the fungal pellet obtained in step (b) with Tris-HCl buffer (pH-6 to 7),
- (d) resuspending the washed fungal pellet in the same buffer in the ratio of 1:4,
- (e) homogenizing and centrifuging the resuspended pellet at 4° C. at 10,000 to 12,000 rpm for 15-30 minutes,
- (f) extracting the supernant of using organic solvents, and
- (g) identifying CPT using conventional means
A process of isolating a novel endophytic fungal strain having accession no. MTCC 5124 from bark of tree Nothapodytes foetida for production of Camptothecin and Camptothecinioids (CPT), said process comprising steps of:
- (a) cutting small stems from tree of Nothapodytes foetida
- (b) disinfecting the stems with ethanol (5-70%),
- (c) removing the outer bark using a sterile blade,
- (d) putting the pieces of inner bark of the stem onto a agar medium
- (e) achieving the growth of endophytic fungus in 2-5 days,
- (f) reculturing the fungus onto a fresh PDA medium for growth and multiplication, and
- (g) identifying the characteristics of the fungal strain.
Dependencies are inferred from the claim text.