Identification of novel MS4A gene family members expressed by hematopoietic cells
OwnerThomas Tedder, Ying Lianginventors
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Abstract
Isolated nucleic acids encoding MS4A polypeptides, isolated MS4A polypeptides, and uses thereof. The disclosed MS4A nucleic acids and polypeptides can be used to generate a mouse model of atopic disorders, for drug discovery screens, and for therapeutic treatment of atopic disorders or other MS4A-related conditions.
Claims19
An isolated MS4A polypeptide, or functional portion thereof, comprising:
- (a) a polypeptide encoded by the nucleotide sequence of any one of the odd-numbered SEQ ID NOs:1-37;
- (b) a polypeptide encoded by a nucleic acid molecule that is substantially identical to any one of the odd-numbered SEQ ID NOs:1-37;
- (c) a polypeptide having the amino acid sequence of any one of the even-numbered SEQ ID NOs:2-38;
- (d) a polypeptide that is a biological equivalent of the polypeptide of any one the even-numbered SEQ ID NOs:2-38;
- or
- (e) a polypeptide which is immunologically cross-reactive with an antibody that shows specific binding with a polypeptide of any one of the even-numbered SEQ ID NOs:2-38.
An isolated nucleic acid molecule encoding a MS4A polypeptide, comprising:
- (a) the nucleotide sequence of any one of the odd-numbered SEQ ID NOs:1-37;
- or
- (b) a nucleic acid molecule substantially identical to any one of the odd-numbered SEQ ID NOs:1-37.
A method for producing an antibody that specifically recognizes a MS4A polypeptide, the method comprising:
- (a) recombinantly or synthetically producing a MS4A polypeptide, or portion thereof;
- (b) formulating the polypeptide of (a) whereby it is an effective immunogen;
- (c) administering to an animal the formulation of (b) to generate an immune response in the animal comprising production of antibodies, wherein antibodies are present in the blood serum of the animal;
- and
- (d) collecting the blood serum from the animal of (c), the blood serum comprising antibodies that specifically recognize a MS4A polypeptide.
A method for identifying a candidate compound as a modulator of MS4A gene expression, the method comprising:
- (a) exposing a cell sample with a candidate compound to be tested, the cell sample containing at least one cell containing a DNA construct comprising a modulatable transcriptional regulatory sequence of a MS4A-encoding nucleic acid and a reporter gene which is capable of producing a detectable signal;
- (b) evaluating an amount of signal produced in relation to a control sample;
- and
- (c) identifying a candidate compound as a modulator of MS4A gene expression based on the amount of signal produced in relation to a control sample.
A method for modulating MS4A function in a subject, the method comprising:
- (a) preparing a gene therapy vector having a nucleotide sequence encoding a MS4A polypeptide or a nucleotide sequence encoding a nucleic acid molecule, peptide, or protein that interacts with a MS4A nucleic acid or polypeptide;
- and
- (b) administering the gene therapy vector to a subject, whereby the function of MS4A in the subject is modulated.
Dependencies are inferred from the claim text.