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US 2006/0134749 A1Published application
Protease, DNA encoding the same, and method for manufacturing protease
AssigneeJellice Co., Ltd.
InventorsYoshitaka Kanayama, Yasuo Sakai, Takahiro Hongo
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Abstract
The present invention provides a protease, DNA encoding this protease, a recombinant vector comprising this DNA, and a method for manufacturing the protease using the transformant.
Claims22
A protease having the properties stated in (i) and (ii) below:
- (i) a molecular weight of 21,000±1,000 (by SDS-PAGE) and
- (ii) specific protease activity cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (1) below, cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (2) below, or cutting the Ser-Leu site of a peptide or protein comprising amino acid sequence (3) below:
- Pro-Gln-Gly-Phe-Gln-Gly-Pro
- (1;
- SEQ ID NO:4)
- Pro-Ala-Gly-Phe-Ala-Gly-Pro
- (2;
- SEQ ID NO:5)
- Gln-Thr-Gln-Ser-Leu-Val-Thr-Pro.
- (3;
- SEQ ID NO:6)
8 more elements
A DNA having the base sequence of bases 1 to 816 or the base sequence of bases 253 to 816 in SEQ ID NO:
- 1.
A protein having the amino acid sequence given by SEQ ID NO:
- 2 or SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.