Heterologous alpha amylase expression in Aspergillus
AssigneeGenencor International, Inc.
InventorsToby Baldwin, Kathleen Clarkson, Nigel Dunn-Coleman, Suzanne Lantz
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Abstract
The present invention relates to the co-expression and production of a heterologous alpha amylase and an endogenous glucoamylase in an Aspergillus strain and enzyme compositions including the same.
Claims24
An Aspergillus fungal strain comprising DNA encoding a glucoamylase and DNA encoding a heterologous alpha amylase having at least 90% sequence identity to SEQ ID NO:
- 3, wherein both the glucoamylase and the heterologous alpha amylase are expressed and secreted by the Aspergillus fungal strain.
An Aspergillus strain comprising a) DNA encoding an endogenous glucoamylase and b) DNA encoding a heterologous alpha amylase having at least 95% sequence identity to SEQ ID NO:
- 3, wherein the Aspergillus strain expresses both the glucoamylase and the heterologous alpha amylase and overproduces the glucoamylase.
A method of producing an alpha amylase in an Aspergillus cell which comprises, culturing an Aspergillus cell which is capable of expressing a heterologous alpha amylase polypeptide having at least 90% sequence identity to SEQ ID NO:
- 3 or SEQ ID NO:
- 4 under conditions suitable for the expression and production of the heterologous alpha amylase and producing the heterologous alpha amylase.
A method for producing an acid stable alpha amylase and a glucoamylase in a filamentous fungal host cell comprising
- a) co-expressing a glucoamylase and an alpha amylase in a filamentous host by transforming the filamentous fungal cell with a DNA construct comprising a promoter having transcriptional activity in the host cell operably linked to a polynucleotide encoding an alpha amylase having at least 90% sequence identity to SEQ ID NO:
- 3, wherein said host cell further expresses and produces a glucoamylase,
- b) cultivating the transformed host cell in a suitable culture medium to allow expression of the alpha amylase and the glucoamylase, and
- c) producing the alpha amylase and the glucoamylase.
Dependencies are inferred from the claim text.