Vectors and methods for enhanced cell longevity and protein expression
AssigneeUniversity of Kentucky Research Foundation
InventorsBruce Webb, Jeremy Kroemer
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Abstract
It is the object of the current invention to provide methods and compositions relating to the expression of vankyrin proteins in cell lines to increase their viability, longevity and capacity for protein production. The inventors have discovered that the expression of P-ank-1 and I2-ank-3 proteins in cell culture has increased the cells' longevity and capacity for endogenous and/or heterologous target protein production. Specifically, the present invention relates to the enhanced expression of endogenous and/or heterologous target proteins/polypeptides in recombinant cells that are also expressing P-ank-1 and/or I2-ank-3 protein compared to expression host cells that are not expressing P-ank-1 and/or I2-ank-3 protein.
Claims22
A vankyrin expression vector comprising a nucleic acid encoding the polypeptide of SEQ ID NO:
- 2.
A vankyrin expression vector comprising a nucleic acid encoding the polypeptide of SEQ ID NO:
- 4.
A vankyrin expression vector comprising a nucleic acid that hybridizes to the nucleic acid of SEQ ID NO:
- 1 under stringent conditions wherein the nucleic acid encodes a polypeptide capable of enhancing longevity and/or protein production of a cell line in which it is expressed.
A vankyrin expression vector comprising a nucleic acid that hybridizes to the nucleic acid of SEQ ID NO:
- 3 under stringent conditions wherein the nucleic acid encodes a polypeptide capable of enhancing longevity and/or protein production of a cell line in which it is expressed.
A vankyrin expression vector comprising a nucleic acid of SEQ ID NO:
- 1.
A vankyrin expression vector comprising a nucleic acid of SEQ ID NO:
- 3.
A recombinant cell comprising a first nucleic acid selected from the group consisting of:
- a. a nucleic acid encoding the polypeptide of SEQ ID NO:
- 2;
- b. a nucleic acid that hybridizes to the nucleic acid of SEQ ID NO:
- 1 under stringent conditions wherein the nucleic acid encodes a polypeptide capable of enhancing longevity and/or protein production of a cell line in which it is expressed;
- and
- c. a nucleic acid of SEQ ID NO:
- 1;
- and/or a second nucleic acid selected from the group consisting of:
- a. a nucleic acid encoding the polypeptide of SEQ ID NO:
- 4;
- b. a nucleic acid that hybridizes to the nucleic acid of SEQ ID NO:
- 3 under stringent conditions wherein the nucleic acidencodes a polypeptide capable of enhancing longevity and/or protein production of a cell line in which it is expressed;
- and
- c. a nucleic acid of SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.