Loading patent…
AssigneeCargill, Incorporated
InventorsBrian Brazeau, Jean-Claude De Troostembergh, Paula Hicks, Holly Jessen, Sara McFarlan, Willy Obyn, Fernando Sanchez-Riera, William Schroeder
Estimating the term…
A method is disclosed for increasing the specific activity of myo-inositol oxygenase. The method includes incubating a mixture including myo-inositol oxygenase and a non-sulfur containing reductant under conditions effective to increase the specific activity of the myo-inositol oxygenase. Also disclosed are methods for producing D-glucuronic acid and glucurono-γ-lactone comprising incubating a mixture including myo-inositol, myo-inositol oxygenase, and oxygen under conditions effective to form 5 grams D-glucuronic acid per liter of the mixture to 400 grams D-glucuronic acid per liter of the mixture. Glucurono-γ-lactone can be produced from the D-glucuronic acid product. Also disclosed are organisms and nucleic acids suitable for use in such methods.
A method for increasing the specific activity of myo-inositol oxygenase, comprising incubating a mixture comprising myo-inositol oxygenase and a non-sulfur containing reductant;
A method for producing D-glucuronic acid, comprising:
An isolated plasmid, said plasmid lacking an antibiotic selectable marker and comprising a Cryptococcus neoformans mio coding sequence that lacks tandem CGA codons for arginine.
A Gram-negative bacterial strain, said strain comprising a Cryptococcus neoformans mio coding sequence that lacks tandem CGA codons for arginine.
Dependencies are inferred from the claim text.