Composition and methods relating to SENP1-a sentrin-specific protease
AssigneesNational Institutes of Health (NIH), The United States of America as represented by the Department of Health and Human Services, U.S. Government NIH Divisional of Extramural Inventions and Technology Resources (DEITR)
InventorsEdward Yeh, Limin Gong
Life of this patent
Estimating the term…
Abstract
The invention relates to a novel protease, called SENP1, which is active against sentrin-modified proteins in vivo. The invention more specifically relates to the genomic and amino acid sequences for SENP1, compositions related to and based on these sequences, and methods of using these sequences and compositions.
Claims65
An isolated and purified polynucleotide comprising a nucleic acid sequence that encodes an amino acid sequence of an SENP1 polypeptide.
An expression vector comprising a polynucleotide comprising a nucleic acid sequence encoding at least 10 contiguous amino acids of SEQ ID NO:2.
A recombinant host cell transfected with a polynucleotide comprising a nucleic acid sequence encoding at least 10 contiguous amino acids of SEQ ID NO:2.
A process of preparing a SENP1 polypeptide comprising:
- transfecting a cell with the polynucleotide comprising all or part of the sequence of SEQ ID NO:1 to produce a transformed host cell;
- and maintaining the transformed host cell under biological conditions sufficient for expression of the polypeptide.
A method of identifying a modulator of a SENP1 polypeptide comprising:
- (a) contacting the SENP1 polypeptide with a candidate substance;
- and
- (b) assaying whether the candidate substance specifically binds to the SENP1 polypeptide.
An isolated and purified nucleic acid sequence that comprises a region encoding at least 14 contiguous bases identical to, or complementary to, SEQ ID NO:1.
An isolated and purified polynucleotide comprising a nucleic acid sequence that encodes an amino acid sequence of an SENP2 polypeptide.
An expression vector comprising a polynucleotide comprising a nucleic acid sequence encoding at least 10 contiguous amino acids of SEQ ID NO:8.
A recombinant host cell transfected with a polynucleotide comprising a nucleic acid sequence encoding at least 10 contiguous amino acids of SEQ ID NO:8.
A process of preparing a SENP2 polypeptide comprising:
- transfecting a cell with the polynucleotide comprising all or part of the sequence of SEQ ID NO:7 to produce a transformed host cell;
- and maintaining the transformed host cell under biological conditions sufficient for expression of the polypeptide.
A method of identifying a modulator of a SENP2 polypeptide comprising:
- (a) contacting the SENP2 polypeptide with a candidate substance;
- and
- (b) assaying whether the candidate substance specifically binds to the SENP2 polypeptide.
An isolated and purified nucleic acid sequence that comprises a region encoding at least 14 contiguous bases identical to, or complementary to, SEQ ID NO:7.
An isolated and purified polynucleotide comprising a nucleic acid sequence that encodes an amino acid sequence of an SENP3 polypeptide.
An expression vector comprising a polynucleotide comprising a nucleic acid sequence encoding at least 10 contiguous amino acids of SEQ ID NO:10.
A recombinant host cell transfected with a polynucleotide comprising a nucleic acid sequence encoding at least 10 contiguous amino acids of SEQ ID NO:10.
A process of preparing a SENP3 polypeptide comprising:
- transfecting a cell with the polynucleotide comprising all or part of the sequence of SEQ ID NO:9 to produce a transformed host cell;
- and maintaining the transformed host cell under biological conditions sufficient for expression of the polypeptide.
A method of identifying a modulator of a SENP3 polypeptide comprising:
- (a) contacting the SENP3 polypeptide with a candidate substance;
- and
- (b) assaying whether the candidate substance specifically binds to the SENP3 polypeptide.
An isolated and purified nucleic acid sequence that comprises a region encoding at least 14 contiguous bases identical to, or complementary to, SEQ ID NO:9.
An isolated sentrin-specific protease (SENP) polypeptide comprising the amino acid sequence PIHL/RXVHW wherein X is a gylcine, lysine or glutamic acid.
An isolated polypeptide comprising a sentrin-specific protease SENP1.
A method of purifying a sentric-specific protease SENP1 polypeptide from a sample comprising collecting a sentric-specific protease polypeptide sample;
- incubating the sample with an antibody that specifically binds the SENP1 polypeptide;
- and obtaining the SENP1 polypeptide.
Dependencies are inferred from the claim text.