Production of functional proteins: balance of shear stress and gravity
AssigneeNASA, Johnson Space Center
InventorsThomas Goodwin, Timothy Hammond, James Kaysen
Life of this patent
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Abstract
The present invention provides for a method of culturing cells and inducing the expression of at least one gene in the cell culture. The method provides for contacting the cell with a transcription factor decoy oligonucleotide sequence comprising a nucleotide sequence encoding a shear stress response element.
Claims21
1-26. (canceled)
A shear stress response element (SSRE) transcription factor decoy oligonucleotide comprising the nucleotide sequence selected from the group consisting of GAGACC and GGTCTC.
A method of producing biomolecules comprising the steps of:
- culturing at least one cell;
- contacting said cell with an SSRE transcription factor decoy oligonucleotide comprising a shear stress response element sequence selected from the group consisting of GAGACC and GGTCTC;
- and
- isolating biomolecules from the cell culture.
A method of producing renal tubular epithelial cells from a cultured cell, comprising the steps of:
- culturing at least one cell;
- contacting said cell with an SSRE transcription factor decoy oligonucleotide comprising a shear stress response element sequence selected from the group consisting of GAGACC and GGTCTC;
- and
- isolating differentiated renal tubular epithelial cells.
Dependencies are inferred from the claim text.