Process for the universal detection of microorganisms and reaction environment permitting the implementation of the process
AssigneeHEMOSYSTEM, a corporation of France
InventorsIsabelle Besson-Faure, Jean-Pierre Hermet, Sebastien Ribault
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Abstract
A process for detecting microorganisms present in a biological fluid including a) contacting a sample of the biological fluid with a reaction environment comprising a marking agent that is a derivative of cyanines and at least one reactant of cellular penetration of the membrane of the microorganisms, b) filtering the sample on a filter capable of retaining the marked microorganisms present in the sample, and c) detecting the marked microorganisms retained in the filter in stage (b).
Claims28
A process for detecting microorganisms present in a biological fluid comprising:
- a) contacting a sample of the biological fluid with a reaction environment comprising a marking agent that is a derivative of cyanines and at least one reactant of cellular penetration of the membrane of the microorganisms,
- b) filtering the sample on a filter capable of retaining the marked microorganisms present in the sample, and
- c) detecting the marked microorganisms retained in the filter in stage (b).
A reaction environment for marking microorganisms, comprising a marking agent that is a derivative of cyanines and at least one cellular penetration agent of the microorganisms.
A process for detecting microorganisms present in a biological fluid comprising:
- a) contacting a sample of the biological fluid with a reaction environment for marking of the microorganisms comprising a marking agent and a reactant of cellular penetration of the membrane of the microorganisms,
- b) filtering the sample on a filter capable of retaining the marked microorganisms present in the sample, and
- c) detecting the marked microorganisms retained in the filter in stage (b).
A cellular penetration reactant comprising:
- PicoGreen at 1/22000 (molecular probes);
- PEI at a final concentration of about 5.5 μg/ml;
- Diacetate chlorohexidine at a final concentration of about 4.5×10−4%;
- N octyl glucopyranoside at a final concentration of about 0.16%;
- Nisine at a final concentration of about 0.018 μg/ml;
- EDTA at a final concentration of about 0.45 mM;
- and
- a buffer saline phosphate (PPS) in a quantity sufficient for a selected final volume.
Dependencies are inferred from the claim text.