Method for the determination of the presence of an antibiotic in a fluid

Abstract

The present invention provides a method for determining the presence or absence of an antibiotic in a fluid comprising: (a) contacting a fluid sample with a test medium comprising a test microorganism and at least one indicator; (b) incubating the test microorganism with the fluid under conditions whereby growth of the test microorganism occurs if no antibiotic is present in the fluid sample; and (c) detecting any growth or inhibition of growth of the test microorganism as appropriate by means of an indicator, characterized in that the ratio of the volume of said fluid sample to the volume of test medium exceeds 2:3 (0.68:1) (v/v). The invention further provides a kit suitable for determining the presence or absence of an antibiotic in a fluid comprising: (a) at least one container partially filled with a test medium comprising a test microorganism, at least one gelling agent and at least one indicator, and; (b) a device for adding fluid to the test medium, said device having a volume that exceeds a ratio of 2/3 (0.68:1) of the volume of the test medium.

Dependencies are inferred from the claim text.