Assay for camp based on exclusive binding of camp to the b-binding sites of camp dependent protein kinase (capk)

A kit for a cAMP assay, said kit comprising a polypeptidic primary binding agent capable of binding cAMP;

• optionally, a labelled cAMP;
• and optionally a secondary binding agent;
• characterized in that said primary binding agent comprises functional cAPK cAMP B-binding sites only.

A polypeptidic cAMP binding agent which comprises functional cAPK cAMP B-binding sites only, and compositions and items comprising said binding agent.

cAMP labelled at the 8-position by iodine-125, and compositions thereof.

13-14. (canceled)

A method for assaying for a cyclic nucleotide or cyclic nucleotide analog, said method comprising contacting said sample with a polypeptidic binding agent capable of binding said cyclic nucleotide or cyclic nucleotide analog and optionally also with a labelled competitor species capable of binding to said binding agent, and detecting conjugates of said binding agent with said cyclic nucleotide or cyclic nucleotide analog or said competitor species, characterized in that said binding agent comprises functional cAPK cAMP B-binding sites only.

A kit for an assay for a cyclic nucleotide or cyclic nucleotide analog, said kit comprising a polypeptidic primary binding agent capable of binding said cyclic nucleotide or cyclic nucleotide analog;

• optionally, a labelled competitor species capable of binding to said binding agent;
• and optionally a secondary binding agent;
• characterized in that said primary binding agent comprises functional cAPK cAMP B-binding sites only.