Electrospun enzyme-nanocomposite biosensing material

Abstract

The present invention provides biosensing material comprising polymer-enzyme nanocomposite fibers. A biosensor comprising such material may be obtained through an electrospinning process to yield a nonwoven mat, which retains enzyme activity. The large amount of available surface area obtained by the methods of the present invention provides unusually high sensitivity and fast response time in sensing applications. Also provided is a biosensing material for monitoring the concentration of an analyte present in a sample, such as urea. The biosensing material contains nanocomposite fibers of an enzyme, such as urease and at least one polymer produced through an electrospinning process. If desired, the enzyme may be encapsulated inside metal oxide semiconductor thin films. A method for preparing the biosensing material through an electrospinning technique is also provided.

Dependencies are inferred from the claim text.