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US 2006/0134712 A1Published application
Multiplexed assay methods
AssigneeMeso Scale Technologies, LLC.
InventorsSelen Stromgren, Eli Glezer
Life of this patent
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Abstract
The present invention is directed to methods for conducting multiplexed assays. The methods are particularly well suited for measuring a plurality of analytes that may be present in very different abundances. The invention also relates to systems, devices, equipment, kits and reagents for use in such methods.
Claims27
A method for measuring analytes in a sample comprising:
- a) contacting a first dilution of said sample with one or more binding surfaces having binding reagents that bind a first analyte and a second analyte;
- b) contacting said binding surface(s) with a first detection reagent, wherein said first detection reagent binds to said first analyte;
- c) contacting a second dilution of said sample with said binding surface(s), said second dilution having a dilution factor less than said first dilution;
- d) contacting said binding surface(s) with a second detection reagent, wherein said second detection reagent binds to said second analyte;
- and
- e) measuring the amount of said first detection reagent and said second detection reagent bound to said binding surface(s).
3 more elements
A method for measuring analytes in a sample comprising:
- a) contacting a fist dilution of said sample with one or mole binding surfaces;
- b) immobilizing an amount of a first analyte on said binding surface(s);
- c) binding a first detection reagent to said first analyte immobilized on said binding surface(s);
- d) contacting a second dilution of said sample with said one or more binding surfaces, said second dilution having a dilution factor less than said first dilution;
- e) immobilizing an amount of a second analyte on said binding surface(s);
- f) binding a second detection reagent to said second analyte immobilized on said surface(s);
- and
- g) measuring amounts of said first detection reagent and said second detection reagent bound to said binding surface(s).
5 more elements
A method for measuring analytes in a sample comprising:
- a) contacting a first dilution of said sample with one or more binding surfaces;
- b) contacting said binding surface(s) with a first detection reagent;
- c) contacting a second dilution of said sample with said binding surface(s), said second dilution having a dilution factor less than said first dilution;
- d) contacting said binding surface(s) with a second detection reagent;
- and
- e) measuring the amount of said first detection reagent and said second detection reagent bound to said binding surface(s).
- wherein
- i) said binding surface(s) and said first detection reagent bind said first analyte;
- ii) said binding surface(s) binds said first analyte and said first detection reagent competes with said first analyte for said binding surface(s);
- iii) said first binding reagent binds said first analyte and said binding surface(s) competes with said first analyte for binding to said first detection reagent;
- and wherein
- i) said binding surface(s) and said second detection reagent bind said second analyte;
- ii) said binding surface(s) binds said second analyte and said second detection reagent competes with said second analyte for said binding surface(s);
- iii) said second binding reagent binds said second analyte and said binding surface(s) competes with said second analyte for binding to said second detection reagent.
11 more elements
A method for measuring analytes in a sample comprising:
- i) contacting a first portion of the sample with one or more binding surfaces;
- ii) immobilizing an amount of a first analyte on said binding surface(s);
- iii) binding a first detection reagent to said first analyte immobilized on the binding surface(s);
- iv) contacting a second portion of the sample with one or more binding surfaces, wherein said second portion has been subjected to different processing compared to said first portion;
- v) immobilizing an amount of a second analyte on the binding surface(s);
- vi) binding a second detection reagent to said second analyte immobilized on said surface(s);
- and
- vii) measuring the amounts of said first detection reagent and said second detection reagent bound to said binding surface(s).
5 more elements
Dependencies are inferred from the claim text.