Atopic dermatitis inducer
OwnerMichihiro Hide, Toshihiko Tanaka +3inventors
InventorsMichihiro Hide, Toshihiko Tanaka, Akio Tanaka, Kaori Ishii, Hidenori Suzuki
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Abstract
An atopic dermatitis inducer binding to a human own IgE antibody and activating mast cells and basophiles, which includes a purified human secretion fraction, or an antigenic molecule or an antigenic determinant in the purified fraction, and obtained through the following steps of: filtering a human secretion, removing insoluble matters and collecting the filtrate; mixing the filtrate with a ConA-affinity carrier and collecting the supernatant; and separating a component having a histamine-releasing activity from the supernatant by column chromatography. This inducer is effective in diagnosing and treating human atopic dermatitis.
Claims17
1-8. (canceled)
A composition activating mast cells and basophils upon binding to a human own IgE antibody and having an atopic dermatitis inducing activity, which is obtained from a human secretion through the following steps of:
- filtering a human secretion, removing insoluble matters and collecting the filtrate;
- mixing the filtrate with a ConA-affinity carrier and collecting the supernatant;
- and
- separating a component having an histamine-releasing activity from the supernatant by column chromatography.
A method of preparing a composition, which is derived from a human secretion, activates mast cells and basophils upon binding to a human own IgE antibody, and has an atopic dermatitis inducing activity, comprising the following steps of:
- filtering a human secretion, removing insoluble matters and collecting the filtrate;
- mixing the filtrate with a ConA-affinity carrier and collecting the supernatant;
- and
- separating a component having an histamine-releasing activity from the supernatant by column chromatography.
Dependencies are inferred from the claim text.